20.500.12556/DKUM-52299 Potential of phenolic antioxidants In vitro studies have shown a link between the consumption of food rich in (poly)phenols, especially flavonoids, and reduced risk of coronary heart diseases. Several flavonoids have been reported to prevent low density lipoprotein cholesterol oxidation in vitro. The aim of our work was to determine antioxidant and radical scavenging activity of flavonoids myricetin,quercetin, rutin, luteolin, apigenin, kaempferol, catechin, epicatechin and epigallocatechin gallate and to study the influence of chemical structure and flavonoid interactions on the ability to inhibit oxidation and scavenge free radicals. Two in vitro methods, i.e. oxidation of â-carotene in an emulsion system and DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical scavenging assay, were applied. In addition, a review of in vivo studies that investigate the effectiveness of food flavonoids in reducing oxidative damage in human body was done and their results were analyzed and compared to in vitro results obtained in our laboratory. Although all tested flavonoids except apigenin showed strong antioxidant and antiradical properties in vitro, these findings can not be completely confirmed from the reviewed in vivo human studies since those results are sometimes contradictoryand inconsistent. Epidemiološke študije kažejo, da ima konzumiranje živil bogatih s polifenoli, predvsem s flavonoidi, preventivni vpliv na koronarno srčno bolezen. Flavonoidi naj bi vplivali na zmanjšanje oksidacije LDL holesterola in vitro. Namen naše študije je bil določiti antioksidativno aktivnost in radikalsko reaktivnost flavonoidov kot so miricetin, kvercetin, rutin, luteolin, apigenin, kempferol, katehin, epikatehin in epikatehin galat ter preučiti vpliv kemijske strukture in interakcij na sposobnost inhibicije oksidacije in vezavo prostih radikalov. Uporabili smo dve in vitro metodi: oksidacijo β-karotena v emulziji in DPPH (1,1-Diphenyl-2-picrylhydrazyl) metodo. V zaključku smo podali pregled ter analizo in vivo študij antioksidativne učinkovitosti flavonoidov v človeškem telesu ter jih primerjali z rezultati naših in vitro raziskav. Čeprav so vsi testirani flavonoidi razen apigenina pokazali močne antioksidativne in antiradikalske lastnosti in vitro, se ti rezultati ne ujemajo popolnoma z in vivo študijami, ki so včasih protislovne in nedosledne. phenolics flavonoids antioxidant activity antiradical activity fenoli flavonoidi antioksidantske aktivnosti antiradicalne aktivnosti true false true Angleški jezik Slovenski jezik Znanstveno delo 2015-07-10 17:22:16 2015-07-10 17:22:16 2022-05-31 03:10:01 2022-03-30 03:07:27 2010 0 0 str. 263-271 št. 2 Letn. 57 2010 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1318-0207 66 14086149 14214678 1318-0207 URN:SI:UM:DK:MPKVRL2W Acta_Chimica_Slovenica_2010_Ruzic,_Skerget,_Knez_Potential_of_phenolic_antioxidants.pdf Acta_Chimica_Slovenica_2010_Ruzic,_Skerget,_Knez_Potential_of_phenolic_antioxidants.pdf 1 9E55B3FE8CF6765B9894B965B0AB4ED2 4d2b0856cf23f48aa686db9e07097c895fc6ca5b7aaed7e43c2b0a34efa51001 8dc5c527-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=115908 http://acta-arhiv.chem-soc.si/57/57-2-263.pdf 1 https://dk.um.si/Dokument.php?lang=slv&id=76859 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0