20.500.12556/DKUM-52576 Introduction and Optimization of the In-Vitro Method for Determining the Hemocompatibility of Modified Poly(Ethylene)Terephthalate Surfaces Vpeljava in optimizacija in-vitro metode za določanje hemokompatibilnosti modificiranih polietilenteraftalatnih površin Purpose: Internationally-accepted standards have been developed for a range of tests and parameters for characterising the in-vitro interactions of biomaterials with blood. However, there are, as yet, no standards concerning the size, design and type of such in-vitro testing systems (1). Since the development of hemocompatible biomaterials provides a very important challenge in material science, there is a need for further progress in finding reliable and standardized methods for hemocompatibility testing (2). The aim of this research was to introduce a method for analyzing the hemocompatibility of different chemically-modified surfaces. Polyethylene terephthalate (PET), with surface modifications using different polysaccharides and their derivatives, were chosen because of their promising biocompatible properties and numerous potential biomedical applications. Methods: A modified hemoglobin-free method was used to determine the antithrombogenicity of the modified PET surfaces (4). The method was optimized for shaking rate, the addition of buffer, and blood temperature to decrease measuring errors. Five differently-modified PET surfaces were analyzed: chemically pre-treated PET and PET treated with chitosan, fucoidan, sulphated chitosan and heparin. Glass was used as a standard thrombogenic surface. Results: The results showed that a lower shaking rate, the addition of buffer, and blood cooling prior to measurement significantly decreased the standard deviation of the measurement results by a total of about 89 %. Conclusions: We believe this optimized hemoglobinfree method is suitable for distinguishing between chemically and structurally-different surfaces, such as glass and PET. The differences between PET surfaces coated with different polysaccharides were, however, less pronounced. Vpeljava in optimizacija in-vitro metode za določanje hemokompatibilnosti modificiranih polietilenteraftalatnih površin. Namen: Razvoj hemokompatibilnih biomaterialov je zelo pomembno področje znanosti o materialih, zato je nujno potreben znaten napredek v iskanju in razvijanju zanesljivih in standardiziranih metod za analizo hemokompatibilnosti ( 2 ). V članku je predstavljena analiza metode sprostitve hemoglobina za določanje časa strjevanja krvi v stiku z obdelanimi površinami PET. Namen raziskave je bil uvedba in optimizacija metode za analizo hemokompatibilnosti različno kemijsko spremenjenih (PET) površin. Metode: Za določanje hemokompatibilnosti različno obdelanih PET površin smo uporabili prilagojeno metodo detekcije prostega hemoglobina (3,4). Analiziranih je bilo pet različno kemično obdelanih PET vzorcev, in sicer: kemijsko predobdelan PET ter PET obdelan s hitosanom, fucoidanom, sulfatiranim chitosanom in heparinom. Steklo je bilo uporabljeno kot standardna trombogena površina. Rezultati: Rezultati pokažejo, da mešanje raztopine hemoglobina in pri 40 tresljajih na minuto zmanjša standardno deviacijo za 68 % v primerjavi s 120 tresljaji na minuto. Dodatek pufra in s tem zvišanje pH iz 5.2 na 7.4 je še dodatno zmanjšalo standardno deviacijo (56 %). Merilno napako smo {e nekoliko zmanj{ali s hlajenjem krvi po odvzemu in naknadnim segrevanjem na 37 oC. Zaključki: Znižanje hitrosti me{anja raztopine, dodatek pufra in ohlajanje krvi pred eksperimentom, značilno zniža standardno deviacijo merilnih rezultatov, in sicer za 89 %. Optimirana metoda in njihovimi derivati niso bile značilne. biomaterials poly(ethyleneterephthalate) hemocompatibility surface modification in-vitro method bioamteriali polietilenteraftalat površinska modifikacija hemokompatibilnost in-vitro metoda true false true Medicinska fakulteta Angleški jezik Slovenski jezik Znanstveno delo 2015-07-10 17:53:21 2015-07-10 17:53:21 2026-03-20 08:20:25 2019-07-11 07:48:22 2009 0 Maribor 0 str. 61-67 št. 1 Letn. 2 2009 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616 242526720 13224470 10.18690/actabiomed.14 1855-5640 URN:SI:UM:DK:F9L8TNBC http://www.dlib.si/details/URN:NBN:SI:doc-AMLJAP7Y 1 https://dk.um.si/Dokument.php?lang=slv&id=77135 RAZ_Stana_Jan_2009.pdf RAZ_Stana_Jan_2009.pdf 1 DCD6FF703E952D7C8D4EB4076ECC1C36 8011b65f73bd1ffb8addf2a59088df2ad54b43b7ac982fb6dec601f263c6342a adc8035d-0d69-11f1-a5b8-00155d580e1a https://dk.um.si/Dokument.php?lang=slv&id=203494 https://journals.um.si/index.php/amb/article/view/2057 0 dd35a7d3-0d68-11f1-a5b8-00155d580e1a https://dk.um.si/Dokument.php?lang=slv&id=203493 Medicinska fakulteta Acta medico-biotechnica 0 0 0