20.500.12556/DKUM-54108 KINETIKA SPROŠČANJA KURKUMINA IZ RAZLIČNIH TRDNIH FORMULACIJ KINETICS OF CURCUMIN RELEASE FROM VARIOUS SOLID FORMULATIONS V diplomski nalogi smo iskali primerne nosilce za vezavo kurkumina. Raziskovali smo sproščanje aktivne substance kurkumina iz različnih trdnih formulacij. Sproščanje smo študirali v mediju pH želodca in mediju pH debelega črevesa v različnih časovnih intervalih. Ker ima kurkumin največjo biološko aktivnost v debelem črevesu, smo iskali tak nosilec, ki bi se minimalno sproščal v želodcu, popolnoma ali vsaj v veliki večini pa bi se sprostil v debelem črevesu. Ugotovili smo, da tristearat ni primeren nosilec za vezavo kurkumina, saj ni prišlo do sproščanja substance. Z vezavo kurkumina na PEG različnih molskih mas smo dobili primeren nosilec. Kurkumin se je popolnoma sprostil tako v mediju pH želodca kakor tudi v mediju pH debelega črevesa. Prišli smo do ugotovitve, da se hitrost sproščanja aktivne substance z naraščajočo verigo PEG zmanjšuje. Ugotovili smo, da nanoenkapsulacija kurkumina ni primerna metoda, saj ne pride do sproščanja substance v želodcu. V debelem črevesu pa je sproščanje minimalno. S koprecipitacijo smo kurkumin vezali na ciklodekstrin. Ta metoda je bila uspešna, saj se je vsa učinkovina sprostila v želodcu. Daleč največjo antioksidativno aktivnost smo dobili pri vzorcu nanoenkapsulacije. The diploma paper is based on searching for appropriate carriers for binding of curcumin. We studied the release of the active substance of curcumin from different solid formulations. The release was studied in the medium gastric pH and the medium pH of the colon in different periods. Since curcumin has its greatest biological activity in the colon, we were searching for a carrier, which would have a minimal release in the stomach, while it would release completely or mainly in the colon. We came to the conclusion that tristearate is not an appropriate carrier for binding with curcumin, since there was no substance release. The binding of curcumin on the PEG of different molecular mass brought us to the appropriate carrier. Curcumin was completely released in the medium gastric pH as well as in the medium pH of colon. We came to the conclusion that the release rate of the active substance decreases with the increased PEG-chain. We discovered that the nanoencapsulation of curcumin is not an appropriate method, since there is no substance release in the stomach, while there is a minimum release in the colon. Via coprecipitation we managed to bind curcumin to cyclodextrin. This method was effective since the whole agent was released in the stomach. By far the largest anti-oxidative activity was observed with the nanoencapsulation sample. kurkumin mikronizacija sproščanje DPPH curcumin micronisation release DPPH true true false [M. Rebernišek] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2015-08-21 17:20:31 2015-10-30 11:03:24 2022-06-04 03:10:44 2021-05-09 03:32:01 2015 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 544.163.3:547.979.4(043.2) 19431446 URN:SI:UM:DK:NOSZW2OO UN_Rebernisek_Maja_2015.pdf UN_Rebernisek_Maja_2015.pdf 1 A22250F9EED6625574E463B839BC2A1D 45888b23e88cce89683c511bdd609fb974a22c22e6747da319e1f46c83607bab 8dd707d9-7c07-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=79314 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0