20.500.12556/DKUM-54251 BIOOGLJE KOT NOSILEC ZA IMOBILIZACIJO ENCIMA BIOCHAR AS A CARRIER FOR ENZYME IMMOBILIZATION Namen diplomske naloge je bil uspešno imobilizirati encim na biooglje, nosilec, ki je bil pripravljen s hidrotermično karbonizacijo odpadne biomase. S spreminjanjem koncentracije encima in z različno izbiro mrežnega povezovalca smo želeli izvesti imobilizacijo encima na biooglje, ki nam da najvišjo preostalo aktivnost encima. Zanimalo nas je, kaj se z aktivnostjo imobiliziranega encima dogaja pri izpostavitvi različnim dejavnikom kot sta temperatura in čas skladiščenja. Rezultati kažejo, da najvišjo preostalo aktivnost obdrži imobiliziran encim s koncentracijo 0,05 g/mL ter ob uporabi mrežnega povezovalca gluteraldehida, koncentracije 2 %. Čas imobilizacije encima je bil 24 ur. Na stresalniku smo uporabili hitrost stresanja 400 rpm. Pri izpostavitvi imobiliziranega encima določeni temperaturi smo ugotovili, da dalj časa je izpostavljen višji temperaturi, bolj bo njegova aktivnost padala. Pri različnem času skladiščenja pa smo prišli do zaključka, da aktivnost encima s časom skladiščenja prav tako pada. The purpose of the thesis was a successful immobilization of enzymes on biochar, a carrier, which was prepared from hydrothermal carbonization processes of biomass. By varying the concentration of the enzyme with a different choice of crosslinking agent, we want to perform immobilization of the enzyme on biochar, which gives the highest residual activity of the enzyme. We wanted to know, what is happening with the activity of the immobilized enzyme, when exposed to different conditions such as temperature and time of storage. The results show that the maximum residual activity of the immobilized enzyme was detected at enzyme concentration of 0.05 g/mL, using the crosslinking agent gluteraldehide, concentration of 2 %. The immobilization time of the enzyme was 24 hours. On a shaker, we used the agitation rate of 400 rpm. During the exposal of immobilized enzyme to a certain temperature, we found out that with the prolongation of exposition time of immobilized enzyme to the higher temperature, the activity of enzyme decreased. It was also detected that with the increase of storage time, the activity of immobilized enzyme decreased. biooglje hidrotermična karbonizacija imobilizacija encima lakaza gluteraldehid biochar hydrothermal carbonization enzyme immobilization laccase gluteraldehid true true false [S. Zadravec] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2015-08-26 16:09:36 2015-10-22 10:35:26 2022-06-05 03:05:33 2021-05-10 03:12:26 2015 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.046.562(043.2) 19428118 URN:SI:UM:DK:B34XRSGN UN_Zadravec_Stasa_2015.pdf UN_Zadravec_Stasa_2015.pdf 1 58BFE31FF18982C870752689EAE76DDC 52a68cf5cc11caf3c332a4640f0be7d6b2b6128013f9105a9ab4973957351b29 09b7eb77-7c08-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=79514 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0