20.500.12556/DKUM-54412 PRIDOBIVANJE MAGNETNIH ZAMREŽENIH ENCIMSKIH SKUPKOV S PRECIPITACIJO Z ETANOLOM SYNTHESIS OF MAGNETIC CROSS-LINKED ENZYME AGGREGATES WITH ETHANOL PRECIPITATION Diplomsko delo zajema pripravo magnetnih zamreženih encimskih skupkov (ali na kratko mCLEAs) iz encima celulaze. Naš namen je bil določiti optimalne pogoje za pripravo mCLEAs tako, da bosta učinkovitost in aktivnost imobilizacije zamreženih encimskih skupkov najvišja. V prvem delu diplomskega dela smo pripravili magnetne zamrežene encimske skupke s precipitacijo z etanolom. Pri tem smo spreminjali različne parametre (hitrost stresanja, čas stresanja, koncentracijo glutaraldehida in temperaturo), medtem ko je bil namen drugega dela diplomskega dela izpostavitev mCLEAs različnim temperaturam (23°C, 4°C, -15°C), ter ugotavljanje, kako se aktivnost mCLEAs pri različnih pogojih izpostavitve spreminja s časom. Naši rezultati prikazujejo, da je bila aktivnost mCLEAs najvišja, ko smo si mCLEAs pripravili pri 10°C in uporabili 0,7 % koncetracijo glutaraldehida. Pri izpostavitvi mCLEAs različnim temperaturam pa smo ugotovili, da aktivnost mCLEAs s časom najbolj upada kadar so bili mCLEAs izpostavljeni pri -15°C. The aim of this work was to prepare the magnetic cross-linked enzyme aggregates (or briefly mCLEAs) of the enzyme cellulase. Our goal was to optimize the preparation of mCLEAs so that the efficiency and activity of the immobilized cross-linked enzyme would be preserved. In the first part of the thesis, we used ethanol precipitation to prepare magnetic cross-linked enzyme aggregates. In this part we tested how different condition (agitation rate, the agitation time, the concentration of glutaraldehyde, and temperature) affect mCLEAS activity. In the second part of the thesis we tested how different temperatures that we used during mCLEAs preparation (23 ° C, 4 ° C, -15 ° C) affect the final mCLEAs activity. Our results show that we obtain the highest final mCLEAs activity when we are working at 10°C and using 0.7% glutaraldehyde. The highest decrease in mCLEAs activity we obtained when mCLEAs were exposed to -15 ° C. etanol glutaraldehid magnetni zamreženi encimski skupki precipitacija zamreženje ethanol glutaraldehyde magnetic cross-linked enzyme aggregates precipitating crosslinking true true false [S. Matis] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2015-09-01 09:52:28 2015-10-22 11:19:53 2022-06-05 03:06:27 2021-05-11 03:34:34 2015 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602.4.577.151.4(043.2) 19424790 URN:SI:UM:DK:3PAUTF35 UN_Matis_Stasa_2015.pdf UN_Matis_Stasa_2015.pdf 1 03DF60FCBD092AD5273ED64CB0CFC195 0892cd9dd88ac3542e7ec3b4d479e51c62d9a21a74fdbc49a3c31b062bafeb71 ac08a0b6-7c07-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=80993 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0