20.500.12556/DKUM-55249 PRIPRAVA BIOKOMPATIBILNIH NANODELCEV ZA UPORABO V MEDICINI PREPARATION OF BIOCOMPATIBLE NANOPARTICLES FOR APPLICATIONS IN MEDICINE Namen magistrskega dela je bil sintetizirati superparamagnetne nanodelce sestave Cu32,5Ni67,5 v matrici silike z ozko porazdelitvijo velikosti s pomočjo sol-gel metode. Za dodatno homogenizacijo in redukcijo oksidov smo sintetiziran vzorec znane sestave segrevali v cevni peči pri temperaturi 850 °C in času 12 ur v redukcijski atmosferi Ar/H2. Nanodelci imajo zadovoljivo Curiejevo temperaturo, ki je primerna za uporabo v samoregulativni magnetni hipertermiji. Želeli smo združiti dva terapevtska pristopa, in sicer magnetno hipertermijo in farmakoterapevtsko zdravljenje. Glavni cilj je bil sintetizirane nanodelce napolniti z zdravilnimi učinkovinami (ZU). ZU (paracetamol, bupivakain hidroklorid in pentoksifilin) smo uspešno vgradili v pore nanodelcev v matrici silike. S pomočjo UV/Vis spektrofotometra smo spremljali sproščanje ZU iz superparamagnetnih nanodelcev. Vsak vzorec smo sproščali v vsaj dveh paralelkah po 180 minut. Pomerjene absorbance smo preračunali v koncentracije oziroma deleže sproščenih ZU po določenih časovnih intervalih. Sintetizirane nanodelce sestave Cu32,5Ni67,5 smo okarakterizirali s pomočjo rentgenske praškovne difrakcije (RTG), termogravimetrične analize (TGA/SDTA), Fourier transformirane infrardeče spektroskopije (FTIR), transmisijske elektronske mikroskopije (TEM) in magnetnih meritev. Površino, volumen in velikost por smo določili z BET analizo. Curiejevo temperaturo smo določili s pomočjo termogravimetrične aparature (TGA/SDTA), ki smo jo opremili s permanentnim magnetom. Superparamagnetni nanodelci so uporabni na področju magnetne hipertermije, kot tudi na področju aktivnega farmakoterapevtskega zdravljenja. The aim of this master's thesis was to synthesize superparamagnetic nanoparticles with the composition Cu32,5N67,5 in a silica matrix with narrow size distribution by sol-gel method. Following additional homogenization and oxidation-reduction, the synthesized specimen of known composition was heated in a tube furnace at 850 °C for 12 hours in an Ar/H2 reduction atmosphere. The nanoparticles possess a satisfactory Curie temperature, suitable for use in self-regulatory magnetic hyperthermia. We wanted to combine two therapeutic approaches: magnetic hyperthermia and pharmaco-therapeutic therapy. The main objective was to charge nanoparticles with active substances. Furthermore, these active substances (paracetamol, bupivacaine hydrochloride and pentoxifylline) were successfully introduced into pores of nanoparticles in silica matrix. We closely followed the release of active substances from superparamagnetic nanoparticles using a UV/Vis spectrophotometer. Every specimen was released in at least two replicates 180 minutes each. Measured absorbance values were converted into concentrations and proportions of released active substances after specified time intervals, respectively. Synthesized nanoparticles with the composition Cu32,5N67,5 were characterized using the X-ray powder diffraction (XRD), thermogravimetric analysis (TGA/SDTA), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and magnetic measurements. The surface, volume and size of pores were defined with the BET analysis. Furthermore, the Curie temperature was determined with the thermogravimetric apparatus (TGA/SDTA), equipped with a permanent magnet. Superparamagnetic nanoparticles are applicable in the field of magnetic hyperthermia, as well as in the field of active pharmaco-therapeutic therapy. CuNi nanodelci sol-gel metoda magnetna hipertermija sproščanje zdravilne učinkovine Curiejeva temperatura. CuNi nanoparticles sol-gel method magnetic hyperthermia drug release Curie temperature. true true false [N. Osenjak] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2015-10-12 13:04:31 2015-12-01 08:04:38 2022-06-08 03:11:23 2021-05-14 03:16:29 2015 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 [544.022.822:620.3]:615(043.2) 19450390 URN:SI:UM:DK:ZM79LLO5 MAG_Osenjak_Nina_2015.pdf MAG_Osenjak_Nina_2015.pdf 1 36DE1E4D4704DC1BA3D57D1D05E412E7 b041eadca9cd14d018cf91c78657749e52fbf8d3555ce2c2a959cbe47ec45fcf 07fb9cff-7c08-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=82853 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0