20.500.12556/DKUM-56893-da8433ea-c184-d0ea-c024-201fc285e642 Hormeza - primer odgovora astrocitov po izpostavljenosti etanolu Hormesis - an example of astrocyte response after ethanol exposure Purpose: In the present work we describe hormesis and review documented hormetic responses in astroglial cells after exposure to different stressors. We present an example of the astrocyte response to acute and chronic ethanol exposure with hormetic characteristics. Methods: As an experimental model, newborn rat cortical astrocytes in culture were used. The cells were exposed to ethanol or the primary metabolite of ethanol (acetaldehyde) for 24 h or 7 days. After treatment, the protein content was determined, and the IL-6 levelsin the culture medium were measured using an enzymelinked immunoassay. Results: Treatment of astroglial cells with ethanol or acetaldehyde led to enhanced protein content and stimulated IL-6 production in the low concentration zone followed by diminished protein content and an inhibition ofIL-6 production at higher concentrations. Chronic exposure of astrocytes wasmore toxic than acute exposure for both compounds, and acetaldehyde was more toxic compared to ethanol. Conclusion: Ethanol and acetaldehyde representstressors for cultured astrocytes and evoke a typical hormetic response after acute and chronic exposure. hormeza astrociti etanol acetaldehid hormesis astrocytes ethanol acetaldehyde true true false Medicinska fakulteta Angleški jezik Slovenski jezik Znanstveno delo 2015-12-30 12:53:26 2015-12-30 12:53:26 2026-03-19 13:27:27 2019-07-11 09:55:03 2012 0 0 str. 65-72 št. 2 Letn. 5 2012 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2012-01-01 577 242526720 512240440 1855-5640 URN:SI:UM:DK:XWHBGNE5 http://www.dlib.si/details/URN:NBN:SI:DOC-JBCXBCS3 1 https://dk.um.si/Dokument.php?lang=slv&id=85099 08.pdf 08.pdf 1 B4399406CAD137A43AE3CCD8B4601FC5 4ec4c178bba763a9c65b29511b541035612c229fface31d460eff7f917761e52 0a1ca534-f8b7-11ee-a0d6-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=178439 Medicinska fakulteta Acta medico-biotechnica 0 0 0