20.500.12556/DKUM-58080 IN VITRO RAZMNOŽEVANJE ŽAJBLJA (Salvia officinalis L.) IN VITRO PROPAGATION OF THE COMMON SAGE (Salvia officinalis L.) V letih 2012–2014 smo v in vitro razmerah s tehniko tkivnih kultur razmnoževali žajbelj (Salvia officinalis L.). V poskus smo vključili izsečke S. officinalis kot izhodiščni material za gojenje v in vitro razmerah ter jih dali na indukcijsko gojišče. Rastlinski material smo z uporabo petih različnih metod sterilizacije predhodno površinsko sterilizirali. Najbolj uspešna je bila sterilizacija pri 15 minutnem obravnavanju s 50% etanolom in DICO. Druga naloga raziskave je bila najti čim bolj primerno gojišče za uspešno razmnoževanje žajblja. Pripravili smo tri različna gojišča G1, G2 in G3, ki so se med seboj razlikovala po vsebnosti rastnih regulatorjev. Največ poganjkov smo namnožili na gojišču G2, ki je vsebovalo BAP v koncentraciji 1 mg/l in IAA v koncentraciji 0,5 mg/l. Tretji del raziskave pa je bil povezan s koreninjenjem. Uporabili smo dve različni gojišči, K1 in K2. Število ukoreninjenih rastlin pri K1 je predstavljalo 82 %, pri K2 pa 79 % vseh rastlin. Na obeh gojiščih smo dobili dobre rezultate. V zaključnem delu raziskave smo rastline aklimatizirali v mini rastlinjakih. Po enem mesecu opazovanja smo prišli do zaključka, da so bile rastline uspešno aklimatizirane. Between 2012 and 2014 we applied the in vitro tissue culture technique to reproduce common sage (Salvia officinalis L.). The experiment involved ordinary cuts/fragments of S. officinalis as starting material for the in vitro cultivation, which was placed on the induction culture medium. The surface of the involved plant material was pre-sterilized using five different approaches. The most successful sterilization involved 50% ethanol and DICA (sodium dichloroisocyanurat) and lasted 15 min. The second task of the experiment was to find the most appropriate medium for sage reproduction. We prepared three different culture media: G1, G2 and G3, each with a different content of growth regulators. Most of the shoots were multiplied in the culture medium G2 that contained BAP (benylamino purine) in concentration of 0.5 mg/l. The aim of the third task was determine the best medium for rooting. We used two different culture media: K1 and K2. The number of rooted plants in the K1 culture medium represents 82% and in K2 79% of all plants. We got good results on both K1 and K2. The last task was the acclimatisation of plantlets in a miniature greenhouse. After a month of observations, we came to the conclusion that the majority of plants were successfully acclimatised. mikropropagacija in vitro tkivne kulture žajbelj Salvia officinalis L micropropagation in vitro tissue culture common sage Salvia officinalis L. true true false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2016-04-06 10:30:44 2016-04-22 14:31:12 2022-06-16 03:06:48 2019-07-11 10:27:52 2016 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 URN:SI:UM:DK:ZWYZEC9O VS_Bencik_Dalia_2016.pdf VS_Bencik_Dalia_2016.pdf 1 3F381F3374015B39DA54F2EE62B0B7F7 1b465bf7fbd7d5d5881ce0dff61ac5a4bf24db9aa01f168a58b448e851a91717 07dccb51-7c0c-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=88069 Fakulteta za kmetijstvo in biosistemske vede 0 0 0