20.500.12556/DKUM-60111 Functional connectivity in islets of Langerhans from mouse pancreas tissue slices We propose a network representation of electrically coupled beta cells in islets of Langerhans. Beta cells are functionally connected on the basis of correlations between calcium dynamics of individual cells, obtained by means of confocal laser-scanning calcium imaging in islets from acute mouse pancreastissue slices. Obtained functional networks are analyzed in the light of known structural and physiological properties of islets. Focusing on the temporal evolution of the network under stimulation with glucose, we show thatthe dynamics are more correlated under stimulation than under non-stimulated conditions and that the highest overall correlation, largely independent of Euclidean distances between cells, is observed in the activation and deactivation phases when cells are driven by the external stimulus. Moreover, we find that the range of interactions in networks during activity shows a clear dependence on the Euclidean distance, lending support to previous observations that beta cells are synchronized via calcium waves spreading throughout islets. Most interestingly, the functional connectivity patterns between beta cells exhibit small-world properties, suggesting that beta cells do not form a homogeneous geometric network but are connected in a functionally more efficient way. Presented results provide support for the existing knowledge of beta cell physiology from a network perspective and shedimportant new light on the functional organization of beta cell syncitia whose structural topology is probably not as trivial as believed so far. islets of Langerhans mouse pancreas Langerhansovi otočki trebušna slinavka miši true false true Angleški jezik Slovenski jezik Znanstveno delo 2016-06-09 03:07:02 2017-06-16 12:02:13 2022-06-22 03:06:03 2019-07-11 11:36:03 2013 0 0 str. 1-12 št. 2 Letn. 9 Feb. 2013 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1553-734X 612.349.7 512883993 512264760 10.1371/journal.pcbi.1002923 1553-734X URN:SI:UM:DK:BGZBJU99 PLoS_Computational_Biology_2013_Stozer_et_al._Functional_Connectivity_in_Islets_of_Langerhans_from_Mouse_Pancreas_Tissue_Slices.PDF PLoS_Computational_Biology_2013_Stozer_et_al._Functional_Connectivity_in_Islets_of_Langerhans_from_Mouse_Pancreas_Tissue_Slices.PDF 1 FD2A5320E9D4122CAE4F9A9470E5CE08 4074f49ed230e657b3a9b2d21a5d09cee428ddba3e4a2b835d5cb39cabd5807b 4120b1cc-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=112657 http://dx.plos.org/10.1371/journal.pcbi.1002923 1 https://dk.um.si/Dokument.php?lang=slv&id=93926 Medicinska fakulteta 0 0 0