20.500.12556/DKUM-60170 Effect of solid state fermentation medium optimization on Pleurotus ostreatus laccase production The objective of this work was to increase laccase production by Pleurotus ostreatus PLAB through culture medium optimization using solid-state culture conditions. Increased laccase activity was obtained through the design of experiments (DOE) using the Taguchi orthogonal array (OA). Seven factors, viz. lignocellulose, glucose, yeast extract, peptone, $KH_2PO_4$, $MgSO_4$ · $7H_2O$ and $MnSO_4$ · $H_2O$ at three levels and pH at two levels with OA layout of L18 ($2^1 × 3^7$) were selected for the proposed experimental design using Minitab 17 software. Data analysis showed that lignocellulose (20%) and glucose (10 g $L^{–1}$) had a positive effect, whereas $KH_2PO_4$, $MgSO_4$ · $7H_2O$ and $MnSO_4$ · $H_2O$ did not have a significant effect on laccase production. Taguchi OA analysis showed that pH 6, lignocellulose 20%, glucose 10 g $L^{–1}$, yeast extract 6 g $L^{–1}$, peptone 15 g $L^{–1}$, $KH_2PO_4$ 3 g $L^{–1}$, $MgSO_4$ · $7H_2O$ 0.5 g $L^{–1}$ and $MnSO_4$ · $H_2O$ 0.1 g $L^{–1}$ were the optimal conditions to maximize laccase production. The model predicted a 30.37 U $g^{–1}$ dry wt., which agreed with the experimentally obtained laccase activity 29.15 U $g^{–1}$ dry wt. at optimal conditions. Namen tega dela je bilo povečanje proizvodnje lakaz glive Pleurotus ostreatus PLAB z optimizacijo gojitvenega medija trdnega gojišča. Povečane aktivnosti lakaz smo pridobili s pomočjo načrtovanja eksperimenta po Taguchi ortogonalni matriki. Izbrali smo sedem faktorjev, od teh so bile vrednosti lignoceluloze, glukoze, kvasnega ekstrakta, peptona, $KH_2PO_4$, $MgSO_4$ · $7H_2O$ in $MnSO_4$ · $H_2O$ izbrane na treh nivojih in vrednosti pH na dveh nivojih. Za načrtovanje eksperimenta smo izbrali predlagano ortogonalno matriko L18 (21 × 37) in programsko opremo Minitab 17. Analiza rezultatov izvedenega eksperimenta je pokazala, da sta imeli lignoceluloza (20 %) in glukoza (10 g $L^{–1}$) pozitivni učinek, medtem ko $KH_2PO_4$, $MgSO_4$ · $7H_2O$ in $MnSO_4$ · $H_2O$ niso imeli pomembnega vpliva na produkcijo lakaz. Analiza rezultatov Taguchi ortogonalne matrike je pokazala, da so pH 6, lignoceluloza 20 %, glukoza 10 g $L^{–1}$, kvasni ekstrakt 6 g $L^{–1}$, pepton 15 g $L^{–1}$, $KH_2PO_4$ 3 g $L^{–1}$, $MgSO_4$ · $7H2_O$ 0,5 g $L^{–1}$ in $MnSO_4$ · $H_2O$ 0,1 g $L^{–1}$ optimalni pogoji za maksimalno produkcijo lakaz. Po modelu je bila napovedana produkcija lakaz 30,37 U $g^{–1}$ suhe snovi pri optimalnih pogojih, eksperimentalno smo dosegli aktivnost lakaz 29,15 U $g^{–1}$ suhe snovi, kar je v skladu s napovedano vrednostjo po modelu. Taguchi method (DOE) orthogonal array optimization Pleurotus ostreatus laccase production solid state fermentation lakaze glive Pleurotus ostreatus fermentacija reakcijski pogoji modeli Taguchi metoda true false true Angleški jezik Slovenski jezik Znanstveno delo 2016-06-09 03:15:27 2017-08-18 10:42:22 2022-06-22 03:06:08 2019-07-11 11:37:38 2015 0 0 str. 932-939 št. 4 Letn. 62 2015 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1318-0207 60:577.152.1 14086149 19268374 10.17344/acsi.2015.1764 1318-0207 URN:SI:UM:DK:S6UURWJK Acta_Chimica_Slovenica_2015_Belsak-Sel_et_al._Effect_of_Solid_State_Fermentation_Medium_Optimization_on_Pleurotus_ostreatus_Laccase_Prod.pdf Acta_Chimica_Slovenica_2015_Belsak-Sel_et_al._Effect_of_Solid_State_Fermentation_Medium_Optimization_on_Pleurotus_ostreatus_Laccase_Prod.pdf 1 E91624088702BD99AD48C9720ED17B7B 5ed9bb32645bdf65bea7b3372f555888a634a5f9f37ea0888a94fbea090a64ec 87310e17-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=115508 https://journals.matheo.si/index.php/ACSi/article/view/1764 1 https://dk.um.si/Dokument.php?lang=slv&id=93985 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0