20.500.12556/DKUM-60193 Covalent binding of heparin to functionalized PET materials for improved haemocompatibility The hemocompatibility of vascular grafts made from poly(ethylene terephthalate) (PET) is insufficient due to the rapid adhesion and activation of blood platelets that occur upon incubation with whole blood. PET polymer was treated with NHx radicals created by passing ammonia through gaseous plasma formed by a microwave discharge, which allowed for functionalization with amino groups. X-ray photoelectron spectroscopy characterization using derivatization with 4-chlorobenzaldehyde indicated that approximately 4% of the –NH2 groups were associated with the PET surface after treatment with the gaseous radicals. The functionalized polymers were coated with an ultra-thin layer of heparin and incubated with fresh blood. The free-hemoglobin technique, which is based on the haemolysis of erythrocytes, indicated improved hemocompatibility, which was confirmed by imaging the samples using confocal optical microscopy. A significant decrease in number of adhered platelets was observed on such samples. Proliferation of both human umbilical vein endothelial cells and human microvascular endothelial cells was enhanced on treated polymers, especially after a few hours of cell seeding. Thus, the technique represents a promising substitute for wet-chemical modification of PET materials prior to coating with heparin. poly(ethylene terephthalate) vascular graft biocompatibility heparin plasma functionalization haemolysis platelet adhesion endothelization žilni vsadki biokompatibilnost heparin plazma funkcionalizacija hemoliza true false true Angleški jezik Slovenski jezik Znanstveno delo 2016-06-09 03:17:01 2017-06-21 09:14:44 2022-06-22 03:06:13 2019-07-11 11:38:23 2015 0 0 str. 1526-1544 št. 4 Letn. 8 2015 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1996-1944 577 33588485 28469543 10.3390/ma8041526 1996-1944 URN:SI:UM:DK:VY41XAFZ Materials_2015_Kolar_et_al._Covalent_Binding_of_Heparin_to_Functionalized_PET_Materials_for_Improved_Haemocompatibility.pdf Materials_2015_Kolar_et_al._Covalent_Binding_of_Heparin_to_Functionalized_PET_Materials_for_Improved_Haemocompatibility.pdf 1 E5BD212AD7F1702BB9D0D2977D1402D0 e60dafa07b828f2b56f6afd758661a2a435a2dbb42a43ea45e2d9140bdb651a3 45ffe795-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=112862 http://www.mdpi.com/1996-1944/8/4/1526/ 1 https://dk.um.si/Dokument.php?lang=slv&id=94008 Fakulteta za strojništvo 0 0 0