20.500.12556/DKUM-60241 SPREMENLJIVOST VSEBNOSTI SKUPNIH PROTEINOV, FENOLOV IN TIOLOV V LISTIH STARIH GENOTIPOV MURV V GORIŠKI REGIJI VARIATIONS IN LEAF TOTAL PROTEIN, PHENOLIC AND THIOL CONTENTS AMONGST OLD VARIETIES OF MULBERRY FROM THE GORIZIA REGION Ohranjanje genskih virov rodu Morus je nezadostno, čeprav je nedvomno bistvenega pomena za ohranjanje svetovne zapuščine za naše naslednike. V magistrskem delu smo popisovali in vzorčili stare genotipe murv v Goriški regiji, ki so jih v preteklosti uporabljali z namenom krme sviloprejkam. Raziskava temelji na določitvi ključnih metabolitov (proteinov, fenolov in tiolov) v listih starih genotipov murv z namenom proučitve antioksidativne aktivnosti in hranilne vrednosti. Skupni proteini in fenoli so bili določeni spektrofotometrično s pomočjo Bradfordove in Folin-Ciocalteu metode, tioli so bili določeni s pomočjo HPLC po predhodnem markiranju s fluorescenčnim barvilom monobromobimane. Skupne proteine smo v listih različnih genotipov merili v razponu od 35 do 126 mg/g SS, skupni fenoli so dosegli vrednosti do 16 g ekvivalent galne kisline/100 g, glutation smo določili v razponu od 705 do 2019 nmol/g SS in cistein od 24 do 73 nmol/g SS. Rezultati določitve metabolitov kažejo, da imajo nekateri genotipi visoko vsebnost glutationa, v primerjavi z rezultati drugih avtorjev so superiorni v vsebnosti proteinov in fenolov in bodo v nadaljnjih raziskavah razmnoženi z namenom uporabe listov za krmo sviloprejk in ostalih živali. Preserving the plant genetic resources of genus Morus is insufficient but undoubtedly vital for conservation of the world’s germplasm for our successors. In the master's thesis we documented and sampled old mulberry genotypes from Gorizia region, that were used as a silkworm’s feed in the past. Research was focused on old mulberry varieties from the Gorizia region in Slovenia which were assessed for their contents on crucial metabolites (proteins, phenolics and thiols) in leaves regarding their antioxidant and nutraceutical potentials. Total proteins were measured spectrophotometrically by following the procedure of Bradford, the total phenolic contents were determined using the Folin-Ciocalteu method. Thiols were marked with monobromobimane fluorescent dye and determined fluorometrically by HPLC. Total proteins in leaves of different mulberry genotypes ranged from 35 to 126 mg/g dry matter (DM), total phenolics reached 16 g gallic acid equivalent/100 g, glutathione varied from 705 to 2019 nmol/g DM and cysteine from 24 to 73 nmol/g DM. The presented metabolite screening showed that some of the evaluated genotypes had higher concentrations of glutathione and were superior in contents of proteins and phenolics when compared to the results of other authors and could be propagated as highly recommendable feed for silkworms, and other animals. krmna rastlina Morus skupni fenoli skupni proteini tioli feeds Morus thiols total phenols total proteins true true false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2016-06-10 17:24:17 2016-06-21 12:12:29 2022-06-22 03:06:28 2019-07-11 11:39:53 2016 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 URN:SI:UM:DK:XL5EBI3L MAG_Ugulin_Tina_2016.pdf MAG_Ugulin_Tina_2016.pdf 1 AF2A592E11F568B64D34D2516E7FB5D5 fd4a10ed3e049b79f30ad86813995234907469cbbb7fbf5fb23f133ca4c80b91 ae172046-7c0c-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=94176 Fakulteta za kmetijstvo in biosistemske vede 0 0 0