20.500.12556/DKUM-60355 VPLIV SESTAVE GOJIŠČA NA USPEŠNOST KORENINJENJA PODLAGE ČEŠNJE GISELA 5 V IN VITRO RAZMERAH THE INFLUENCE OF MEDIUM COMPOSITION ON ROOTING SUCCESS OF GISELA 5 ROOTSTOCK IN IN VITRO CONDITIONS V poskusu, ki smo ga opravljali od junija 2009 do junija 2011 na Fakulteti za kmetijstvo in biosistemske vede v Mariboru, smo želeli sestaviti optimalno gojišče za uspešno koreninjenje podlage Gisela 5 v in vitro razmerah. Kot izhodiščni material smo uporabili poganjke, ki so že rasli in vitro. Najprej smo namnožili 175 poganjkov, ki smo jih prenesli na različna gojišča za koreninjenje. Lepo razvite in zdrave poganjke smo prestavili na sedem gojišč za koreninjenje (K1, K2, K3, K4, K5, K6, K7), ki so vsebovala osnovno gojišče MS z vitamini v različnih koncentracijah, saharozo in različne koncentracije rastnih regulatorjev iz skupine avksinov (IBA in NAA). Kot najboljše se je izkazalo gojišče K5 z 0,5 mg/l IBA, na katerem je koreninilo 71,43 % rastlin. Najslabše pa je bilo gojišče K4 s 3,48 mg/l IBA, na katerem je koreninilo 8,33 % rastlin. Rastline, ki so koreninile, smo presadili v substrat in jih dali aklimatizirat v meglilno komoro v rastlinjak in rastno komoro v laboratoriju. Kot boljša se je izkazala aklimatizacija v meglilni komori v rastlinjaku. The aim of the experiment, which was carried out from June 2009 to June 2011 at the Faculty of Agriculture and Life Sciences in Maribor, was to compose an optimum medium to enable successful rooting of Gisela 5 rootstock in in vitro conditions. As the starting material we used sprouts which had already been growing in vitro. At first we multiplied 175 sprouts which were then carried to different mediums for rooting. Nicely developed and healthy looking sprouts were then moved to seven different mediums for rooting (K1, K2, K3, K4, K5, K6, K7), which all contained the basic MS medium with vitamins in seven different concentrations, sucrose and different concentrations of growth regulators from the group of auxins (IBA and NAA). The best turned out to be K5 medium with 0.5 mg/l IBA which resulted in 71.43 % of rooted plants. On the other hand, the worse turned out to be K4 medium with 3.48 mg/l IBA in which only 8.33 % of plants developed roots. Consequently, the rooted plants were transplanted into the substrate and were acclimatized in the growth chamber in the laboratory and fog chamber in the greenhouse. Acclimatization in the fog chamber in the greenhouse was better compared to the growth chamber češnje in vitro razmnoževanje koreninjenje gojišče Gisela 5 cherry in vitro propagation rooting medium Gisela 5 rootstock true true false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2016-06-16 10:08:06 2016-06-24 10:28:14 2022-06-23 03:10:17 2019-07-11 11:43:40 2016 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 URN:SI:UM:DK:RQYUXUL6 VS_Plohl_Andreja_2016.pdf VS_Plohl_Andreja_2016.pdf 1 2E3E75CD2FADDB80827D06F7986C2FF4 9978bdc7750037da960b26c4bd17410bc14995349df9bf47c9476d55726a7f63 b8fa9e43-7c0c-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=94778 Fakulteta za kmetijstvo in biosistemske vede 0 0 0