20.500.12556/DKUM-61136 Vpliv velikosti vcepka in gojišča na regeneracijo brstov rebrinčevolistne hladnikovke (Hladnikia pastinacifolia Rchb.) v tkivni kulturi The effect of explant size and growth media on regeneration of Hladnikia pastinacifolia Rchb. in tissue culture Rebrinčevolistna hladnikovka (Hladnikia pastinacifolia Rchb.) je redka in potencialno ogrožena endemična vrsta, vključena v program krioprezervacije. Cilj diplomske naloge je določiti najbolj optimalno velikost vcepka, ki bi bila primerna za postopke krioprezervacije in najprimernejša gojišča, za regeneracijo takšnih vcepkov. Raziskali smo tudi učinek predpriprave pri 5 °C na regeneracijo vcepkov. Uporabili smo tri različne velikosti vcepkov brez predhodne krioprezervacije, in sicer majhne (0,5–1 mm), srednje (1–2 mm) in velike (2–3 mm). Le-te smo vcepili na tri različna gojišča, ki jih uporabljamo pri postopkih mikropropagacije, in sicer osnovno MS; osnovno MS z 2 μM BAP (6-benzilamino purin); osnovno MS z 5 μM BAP in 3 μM IBA (indol–3–maslena kislina). Velikost vcepka je vplivala na preživetje in ponovno rast vcepkov. Najboljšo regeneracijo v brste smo dosegli pri velikih vcepkih na osnovnem gojišču MS, medtem ko smo najslabše rezultate dobili pri malih vcepkih. Vcepki na gojiščih z dodanimi rastnimi regulatorji so večinoma regenerirali v kalus ali propadli. Predpriprava je pozitivno vplivala na preživetje vcepkov, vendar je učinke predpriprave potrebno še dodatno raziskati. Hladnikia pastinacifolia Rchb. is a rare and potentially endangered endemic species, involved in the program of cryopreservation. The purpose of the project is to investigate the influence of different culture media on the regeneration of different sizes of explants. Our goal is to determine the optimal size of the explant, which would still be appropriate for the procedures of cryopreservation. We are also investigating the most suitable culture medium for the regeneration of shoot tips. We have also studied the effects of pretreatment in a cool place at 5°C. We have used three different sizes of shoot tips: small (0,5–1 mm), medium (1–2 mm) and large (2–3 mm), which were moved to the different culture media, namely a basic MS medium; MS medium with added 2μM BAP (6-benzylaminopurine) and MS medium with added 5μM BAP and 3μM IBA (indole-3-butyric acid). The size of the explant is effecting on the survival and regrowth of them. The best survival and regrowth rate was achieved in large explants with basic MS medium, while small ones gave the worst results. Explants cultured on medium with added growth regulators usually regenerated callus or they died. Pretreatment had also a positive impact on survival of explants but it needs to be further investigated. Hladnikia pastinacifolia Apiaceae tkivna kultura krioprezervacija velikost vcepka vršiček poganjka Hladnikia pastinacifolia Apiaceae tissue culture cryopreservation true true false [J. Mujakić] Slovenski jezik Angleški jezik Diplomsko delo 2016-07-23 16:32:13 2016-08-19 12:26:35 2022-06-25 03:07:52 2019-07-11 12:10:19 2016 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 57(043.2) 22439944 URN:SI:UM:DK:FWNGG91M UN_Mujakic_Jakup_2016.pdf UN_Mujakic_Jakup_2016.pdf 1 2B526BDE789A10213680834AAF66B26E be2c723d8ad8b824fcb1e4ee994d54d82529640fbe19d5a4ecb23d60cad06532 ef5b5ce9-7c0c-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=97109 Fakulteta za naravoslovje in matematiko 0 0 0