20.500.12556/DKUM-62728 DOLOČANJE LOKALNE GENSKE EKSPRESIJE V POSTOPKU CELJENJA RAN DETERMINATION OF LOCAL GENE EXPRESSION IN THE PROCESS OF WOUND HEALING Nekatere rane, definirane kot kronične rane, se v daljšem časovnem obdobju ne zacelijo. Njihovo celjenje je ustavljeno v vnetni fazi, na kar vpliva več dejavnikov, na primer slaba cirkulacija krvi, visoka starost, težave s premikanjem, razne bolezni, ki zavirajo delovanjo imunskega sistema. V kroničnih ranah se veliko bolj kot v akutnih izražajo vnetni citokini, ki poskušajo odpraviti intenzivno vnetje v rani. Med temi citokini so: interlevkini (IL), interferoni in tumor zavirajoči faktor (TNF). Vrednotenje celjenja rane je bilo do sedaj omejeno bodisi na presojo zdravnika ali pa na merjenje kvalitativnih podatkov o celjenju, medtem ko so poskusi določanja poteka celjenja na kvantitativen, napovedno veliko boljši, način, še v povojih. V diplomskem delu smo se zato lotili vrednotenja celjenja prav na tej osnovi, torej poiskati kvantitativno merljive količine, ki jih lahko povežemo s celjenjem. Osredotočili smo na analizo izražanja vnetnih in imunskih genov: IL4, IL5, IL8, IL10 in CSF2 v kronični diabetični razjedi, kot modelni rani. Rezultati so pokazali, da se vsi geni najbolj izražajo na robovih rane in vedno manj proti sredini. To nakazuje na smer celjenja rane, in sicer od njenega roba proti sredini, kar je v skladu tudi s siceršnjimi opazovanju celjenja. Rezultati so zelo vzpodbudni in so osnova za nadaljnje preiskovanje omenjenega pristopa kot možnega modernega protokola za oceno celjenja ran. Some wounds, defined as chronic wounds, do not heal through long period of time. Their healing is inhibited in the inflammatory phase and influenced by several factors, such as poor blood circulation, high age, difficulty moving and various diseases that suppress the immune system. In chronic wounds the inflammatory cytokines are much more expressed than in acute wounds. Among these cytokines are interleukins (IL), interferons and tumor necrosis factor (TNF). Evaluation of wound healing has so far been limited either to the physician's judgment, or the measurement of qualitative data about healing, while attempts to determine a healing process in a quantitative, predictive much better way, are still in infncy. In this thesis, therefore, we have undertaken the evaluation of healing on this basis, to find quantitatively measurable quantities. We focused on the analysis of the expression of inflammatory and immune genes: IL4, IL5, IL8, IL10 and CSF2 in chronic diabetic ulcer as a model wound. The results showed that are all genes most expressed at the edges of the wound and less expressed towards the center. This indicates the direction of the wound healing from its edges to the center of the wound, which is in line with other observations of wound healing. The results are very encouraging and are the basis for further investigation of approach as a possible modern protocol for evaluation of wound healing. celjenje ran kronične rane vnetje citokini vnetni in imunski geni wound healing chronic wounds inflammation cytokines inflammatory and immune genes true true false [B. Grabrovec] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2016-08-31 13:46:43 2016-10-04 15:26:40 2022-06-30 03:07:25 2019-07-11 13:05:54 2016 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.262.1:616'002(043.2) 20299542 URN:SI:UM:DK:VSJT7N2G UN_Grabrovec_Barbara_2016.pdf UN_Grabrovec_Barbara_2016.pdf 1 EC101AC52AE8C536475B1066E209C4FC b6c013c4eb47e3f9dd5025daac5ced1de1534751ee2299f82ff163cbad498ba6 73cc4518-7c0d-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=104314 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0