20.500.12556/DKUM-63899 MOŽNOSTI MIKROPROPAGACIJE PRAVEGA KOSTANJA (Castanea sativa Mill.), In vitro propagation of european chesnut (Castanea sativa Mill.) Pravi kostanj (Castanea sativa) je lesnata rastlina, ki se težje razmnožuje tako generativno s semenom kot vegetativno s cepljenjem ali potaknjenci. Uspešno razmnoževanje v in vitro razmerah pa je odvisno od matičnih rastlin, sterilizacije, sestave gojišč, natančnosti in pogojev gojenja. V poskus mikropropagacije so bili vključeni 3 genotipi (G1, G2 in G3) iz območja Kozjaka. Za sterilizacijo brstov smo uporabili 6 metod, od katerih se je kot najboljša izkazala sterilizacija s 3 % dikloroizocinaurično kislino (DICA) (72 % sterilnih in vitalnih brstov). Primerjava sterilizacije odprtih in zaprtih brstov je pokazala, da so se bolje sterilizirali zaprti brsti, medtem ko so po uspešni sterilizaciji bolje odgnali odprti brsti. Genotip ni imel večjega vpliva na uspešnost sterilizacije. Dodatek askorbinske kisline v gojišče ni imel pozitivnega učinka na rast kulture. Najuspešnejše gojišče za rast poganjkov pa je bilo MS-NO3 i. Chestnut is a woody plant, known by its difficulties to propagate either generatively with seeds or vegetatively with cuttings or grafting. Successful in vitro propagation depends on mother plants, sterilization, media composition, precision of the propagator and growing conditions. We in vitro propagated 3 genotypes (G1, G2 and G3) from Kozjak area. We tested 6 different methods of bud sterilization to determine the optimal process which was sterilization with 3% dichloroisocyanuric acid (DICA) (72% sterile and vital buds). When we compared sterilization of opened and closed buds, the latter proved to be more successful whereas subsequent growth was better on opened buds. Genotype did not have any significant influence on sterilization. Addition of ascorbic acid in medium did not have any positive influence on the growth of the culture. The best medium for shoot growth was MS-NO3 i. mikropropagacija/ pravi kostanj/ Castanea sativa/ tkivne kulture/ in vitro micropropagation/ European chestnut/ Castanea sativa/ tissue culture/ in vitro propagation true true false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2016-09-19 10:00:09 2016-10-11 07:10:09 2022-07-04 03:05:48 2019-07-11 13:43:31 2016 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 URN:SI:UM:DK:KF0HQEFK UN_Viher_Marko_2016.pdf UN_Viher_Marko_2016.pdf 1 EF8279961B48F3911F0D6AE363057D94 44e3d36cc0bceffe446d0610e2cae3775fe02a31d7b4bbe3a02ac5f5c59b6a82 86f19120-7c0d-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=105147 Fakulteta za kmetijstvo in biosistemske vede 0 0 0