20.500.12556/DKUM-64805 Toksičnost nanodelcev na bazi cinkovega oksida pri izpostavitvi človeških epitelnih in makrofagnih celic Zinc oxide based nanoparticles and their toxicity upon exposure of human epithelial and macrophage cells V magistrski nalogi smo testirali 27 vrst nanodelcev cinkovega oksida (ZnO) glede toksičnosti in vitro. V ta namen smo izmerili vpliv nanodelcev različnih velikosti in oblik na proliferacijo normalnih črevesnih epitelnih celic linije H4 in makrofagne linije TLT. Z eksperimentom smo želeli dokazati, da je toksičnost nanodelcev ZnO na celice odvisna od velikosti, oblike in njihove koncentracije. Preživetje celic po izpostavitvi nanodelcem smo ugotavljali s testom kristal vijolično in reagentom MTT. S programom GraphPad Prism smo določili inhibitorno koncentracijo nanodelcev ZnO, pri kateri dosežemo 50-odstotni inhibitorni učinek glede na kontrolo (IC50). Ugotovljene IC50 vrednosti so bile v razponu do 1,295 mg/l do141,2 mg/l za celice H4 in od 1,467 mg/l do 190,9 mg/l za celice TLT. Ugotovili smo, da so za isto vrsto nanodelcev imele celice TLT v 12-ih primerih nižje vrednosti (višja toksičnost) IC50 kot celice H4, v 15-ih primerih pa višje vrednosti. S programom SPSS korelacije med obliko in toksičnostjo nanodelcev nismo zaznali. Ugotovili smo, da obstaja korelacija med velikostjo in toksičnostjo; večji kot so delci manjša je vrednost IC50. In the present master's thesis we analysed the in vitro toxicity of 27 types of zinc oxide (ZnO) nanoparticles. For this purpose we analysed the effect of nanoparticles of different sizes and shapes on the proliferation of normal cells of the H4 intestinal epithelial cell line and TLT macrophage cells. Our objective was to prove that the toxicity of the ZnO based nanoparticles depends on their size, shape and concentration. We determined the survival of cells after exposure to the nanoparticles by performing a crystal violet test, as well as with the use of the MTT reagent. Using the GraphPad Prism programme we determined the inhibitory concentration of ZnO nanoparticles on viability of cells at which we obtained a 50 percent inhibitory effect in relation to the control (IC50). The IC50 values were in the range between 1.295 mg/l to 141.2 mg/l for H4 cells and from 1.467 mg/l to 190.9 mg/l for TLT cells. Using the same type of nanoparticles we found that in 12 cases TLT cells had a lower IC50 value (higher toxicity) than H4 cells and in 15 cases the values were higher. Using the SPSS programme a significant correlation between the shape and toxicity of nanoparticles has not been detected. It can be concluded that there is a negative correlation between the size and toxicity, since larger particles were less toxic. nanodelci ZnO nanodelci toksičnost H4 TLT IC50 ZnO nanoparticles nanoparticles toxicity H4 TLT IC50 true true false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2016-12-14 15:17:56 2016-12-23 13:19:00 2022-07-06 03:06:40 2019-07-11 14:56:32 2016 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 URN:SI:UM:DK:KD6EJ5BR MAG_Drevensek_Tea_2016.pdf MAG_Drevensek_Tea_2016.pdf 1 5AF1BEE0824DCCB4BA8DF5FBB193FC03 96654397ff17a6633cfa4bff5fc3b3f14f894c6325b51a478b7d215089a96aa7 e4493a33-7c0d-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=108686 Fakulteta za kmetijstvo in biosistemske vede 0 0 0