20.500.12556/DKUM-65293 Micropropagation of the narrow endemic Hladnikia pastinacifolia (Apiaceae) The monotypic Hladnikia pastinacifolia Rchb. is a narrow endemic species, with an extremely small distribution area in Slovenia, prone to any kind of threat that could lead to species extinction. Tissue culture techniques are proposed as a conservation measure for rapid propagation and ex-situ conservation. Tissue culture was initiated from seeds and juvenile plants obtained from natural sites on a solid Murashige and Skoog (MS) medium, with and without growth regulators. We tested various combinations and concentrations of growth regulators, and the best proliferation of axillary shoots, on average 14, was obtained on MS medium with 5 μM BAP and 3 μM IBA and 3% sucrose. Rooting was achieved after transferral of the shoots to an MS medium with 2 μM IBA and 3% sucrose. The rooted plants were acclimatized on a mixture of limestone sand, potting soil and vermiculite in a ratio of 10:2:2, with pH in the range of 7.5–8.0. In vitro propagation methods provide an important opportunity for the propagation and preservation of H. pastinacifolia by rapidly increasing the number of plants, without disturbing the wild population. biotechnology micropropagation micropropagation tissue culture Hladnikia pastinacifolia Apiaceae biotehnologija zaščita mikropropagacija tkivna kultura Hladnikia pastinacifolia Apiaceae true true true University of Zagreb, Faculty of Science, Department of Biology Angleški jezik Slovenski jezik Znanstveno delo 2017-03-28 14:26:02 2017-03-28 14:27:35 2025-09-18 11:53:26 2019-07-11 15:13:44 2016 0 0 Str. 244-252 št. 2 Letn. 75 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 0365-0588 582.79 6134786 22339592 10.1515/botcro-2016-0028 0365-0588 URN:SI:UM:DK:NQEVMDXB Acta_Botanica_Croatica_2016_Ambrozic-Dolinsek,_Ciringer,_Kaligaric_Micropropagation_of_the_Narrow_Endemic_Hladnikia_pastinacifolia_(A(2).pdf Acta_Botanica_Croatica_2016_Ambrozic-Dolinsek,_Ciringer,_Kaligaric_Micropropagation_of_the_Narrow_Endemic_Hladnikia_pastinacifolia_(A(2).pdf 1 B0990AAA69BBC404D60E0F3877D90931 02fc40ea442341b064b24723202daa4e8f58ae6e816b4eae3c78dd70a8a1a180 089a80a2-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=110298 http://www.degruyter.com/view/j/botcro.2016.75.issue-2/botcro-2016-0028/botcro-2016-0028.xml 1 https://dk.um.si/Dokument.php?lang=slv&id=110297 Fakulteta za naravoslovje in matematiko 0 0 0