20.500.12556/DKUM-65442 Single nucleotide polymorphisms in genes MACC1, RAD18, MMP7 and SDF-1[alpha] as prognostic factors in resectable colorectal cancer Background: Colorectal cancer (CRC) represents one of the most common malignancies worldwide. Research has indicated that functional gene changes such as single nucleotide polymorphism (SNP) influence carcinogenesis and metastasis and might have an influence on disease relapse. The aim of our study was to evaluate the role of SNPs in selected genes as prognostic markers in resectable CRC. Patients and methods: In total, 163 consecutive patients treated surgically for CRC of stages I, II and III at the University Medical Centre in Maribor in 2007 and 2008 were investigated. DNA was isolated from formalin-fixed paraffin-embedded CRC tissue from the Department of Pathology and SNPs in genes SDF-1alpha, MMP7, RAD18 and MACC1 were genotyped using polymerase chain reaction followed by high resolution melting curve analysis or restriction fragment length polymorphism. Results: We found worse disease-free survival (DFS) for patients with TT genotype of SNP rs1990172 in gene MACC1 (p = 0.029). Next, we found worse DFS for patients with GG genotype for SNP rs373572 in gene RAD18 (p = 0.020). Higher frequency of genotype GG of MMP7 SNP rs11568818 was found in patients with T3/T4 stage (p = 0.014), N1/N2 stage (p = 0.041) and with lymphovascular invasion (p = 0.018). For MACC1 rs1990172 SNP we found higher frequency of genotype TT in patients with T3/T4 staging (p = 0.024). Higher frequency of genotype GG of RAD18 rs373572 was also found in patients with T1/T2 stage with disease relapse (p = 0.041). Conclusions: Our results indicate the role of SNPs as prognostic factors in resectable CRC. single nucleotide polymorphism colorectal cancer MACC1 RAD18 MMP7 SDF-1a enonukleotidni polimorfizem kolorektalni rak MACC1 RAD18 MMP7 SDF-1a true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-04-05 08:38:07 2017-04-05 08:39:33 2022-07-08 03:08:18 2019-07-11 15:18:19 2016 0 0 9 str. 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1581-3207 616.3-006 784507 512631096 10.1515/raon-2016-0043 1581-3207 URN:SI:UM:DK:LPWAMNDT Radiology_and_Oncology_2016_Horvat_et_al._Single_nucleotide_polymorphisms_in_genes_MACC1,_RAD18,_MMP7_and_SDF-1a_as_prognostic_factors_i.pdf Radiology_and_Oncology_2016_Horvat_et_al._Single_nucleotide_polymorphisms_in_genes_MACC1,_RAD18,_MMP7_and_SDF-1a_as_prognostic_factors_i.pdf 1 7F0D6D63902CC3C7A179B2D3FA5476F4 bd6c2399c5889c97a518b1b9deaa4048926176ce0f29528945daf01071be770c 11730f19-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=110650 http://www.degruyter.com/view/j/raon.ahead-of-print/raon-2016-0043/raon-2016-0043.xml 1 https://dk.um.si/Dokument.php?lang=slv&id=110649 Medicinska fakulteta 0 0 0