20.500.12556/DKUM-65639 VZPOSTAVITEV TKIVNE KULTURE ČESNA (Allium sativum L.) 'PTUJSKI SPOMLADANSKI' ESTABLISHMENT OF GARLIC (Allium sativum L.) 'PTUJSKI SPOMLADANSKI' MERISTEME TISSUE CULTURE V letih 2015 in 2016 smo na Fakulteti za kmetijstvo in biosistemske vede Univerze v Mariboru proučevali metode razmnoževanja česna (Allium sativum L.), in sicer sorto 'Ptujski spomladanski' v in vitro razmerah. Česnove čebulice smo sterilizirali z 20 minutnim tretiranjem s 4 % NaOCl. Iz steriliziranih česnovih čebulic smo pod aseptičnimi pogoji izrezali meristeme in jih ponovno sterilizirali s 4 % NaOCl (15 minut). Ta sterilizacija je bila uspešna za 93 % meristemov. Za regeneracijo poganjkov smo uporabili gojišče MS z vitamini in 8 % saharoze. Ugotovili smo, da dodana jasmonska kislina v koncentraciji 5 µM pozitivno vpliva na nastajanje novih česnovih čebulic. Iz začetnih 32 poganjkov smo v dveh mesecih namnožili 67 poganjkov in 55 česnovih čebulic. Česen je koreninil na gojiščih za subkultiviranje, brez dodanih avksinov, kjer je 54 % rastlin razvilo več kot 10 korenin. Le 6 % rastlin na gojišču za subkultiviranje ni razvilo korenin. V treh tednih smo uspešno aklimatizirali 66 % rastlin. In 2015 and 2016, the methods of garlic (Allium sativum L.) 'Ptujski spomladanski' in vitro reproduction were studied at the Faculty of Agriculture and Life Sciences of the University of Maribor. Garlic cloves were sterilized by a 20-minute treatment with a 4 % sodium hypochlorite. The meristems were cut from the sterilized cloves under aseptic conditions and then re-sterilized with a 4 % sodium hypochlorite (15 minutes). This sterilization was successful for 93 % of meristems. MS medium with vitamins and 8 % of the sucrose were used for the regeneration of the sprouts. It was found that the addition of the jasmonic acid at the concentration of 5 µM had a positive effect on the creation of new cloves. The initial 32 sprouts were multiplied to 67 sprouts and 55 cloves. The garlic developed roots in the subcultivation media, without added auxins, and 54 % of the plants developed more than 10 roots. Only 6 % of the plants in the subcultivation medium did not develop roots. In three weeks, 66 % of the plants were successfully acclimated. mikropropagacija in vitro tkivne kulture česen Allium sativum micropropagation in vitro tissue culture garlic Allium sativum true true false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2017-05-12 07:41:17 2017-05-26 12:00:48 2022-07-09 03:09:45 2019-07-11 15:25:04 2017 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 URN:SI:UM:DK:UFHTMBGY MAG_Zolnir_Erika_2017.pdf MAG_Zolnir_Erika_2017.pdf 1 092CB2BAF484B1474BBFAF00F1933114 122021ca280f6dc38349e61217c01f6d2376749cc585084fa4f47375d0f69d72 1c7847ea-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=111139 Fakulteta za kmetijstvo in biosistemske vede 0 0 0