20.500.12556/DKUM-66186 Tylosema esculentum (Marama) tuber and bean extracts are strong antiviral agents against rotavirus infection Tylosema esculentum (marama) beans and tubers are used as food, and traditional medicine against diarrhoea in Southern Africa. Rotaviruses (RVs) are a major cause of diarrhoea among infants, young children, immunocompromised people, and domesticated animals. Our work is first to determine anti-RV activity of marama bean and tuber ethanol and water extracts; in this case on intestinal enterocyte cells of human infant (H4), adult pig (CLAB) and adult bovine (CIEB) origin. Marama cotyledon ethanolic extract (MCE) and cotyledon water extract (MCW) without RV were not cytotoxic to all cells tested, while seed coat and tuber extracts showed variable levels of cytotoxicity. Marama cotyledon ethanolic and water extracts (MCE and MCW, resp.) (≥0.1 mg/mL), seed coat extract (MSCE) and seed coat water extract (MSCW) (0.01 to 0.001 mg/mL), especially ethanolic, significantly increased cell survival and enhanced survival to cytopathic effects of RV by at least 100% after in vitro co- and pre-incubation treatments. All marama extracts used significantly enhanced nitric oxide release from H4 cells and enhanced TER (Ω/cm2) of enterocyte barriers after coincubation with RV. Marama cotyledon and seed coat extracts inhibited virion infectivity possibly through interference with replication due to accumulation of nitric oxide. Marama extracts are therefore promising microbicides against RV. tylusema esculentum marama antiviral agents rotavirus tylosema esculentum marama protivirusna sredstva rotavirus true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-06-14 10:50:14 2017-06-14 10:51:52 2022-07-10 03:06:45 2021-05-15 03:27:13 2011 0 0 Str. 1-11 Letn. 2011 2011 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1741-427X 635.654:579.61:615.32 513423897 3094572 10.1155/2011/284795 1741-427X URN:SI:UM:DK:1IZ5S5Z9 Evidence-Based_Complementary_and_Alternative_Medicine_2011_Chingwaru_et_al._Tylosema_esculentum_(Marama)_Tuber_and_Bean_Extracts_Are_Str.pdf Evidence-Based_Complementary_and_Alternative_Medicine_2011_Chingwaru_et_al._Tylosema_esculentum_(Marama)_Tuber_and_Bean_Extracts_Are_Str.pdf 1 BCDB29AC632C17E1D0278575E6A34A1B 4ff40f84b9efa0e0857c108d99cd32ae56e9ab8283e00eb923b5138d7a612a5e 3da65e99-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=112500 http://www.hindawi.com/journals/ecam/2011/284795/ 1 https://dk.um.si/Dokument.php?lang=slv&id=112499 Fakulteta za kmetijstvo in biosistemske vede 0 0 0