20.500.12556/DKUM-66201 Effect of base sequence on G-wire formation in solution The formation and dimensions of G-wires by different short G-rich DNA sequences in solution were investigated by dynamic light scattering (DLS) and polyacrilamide gel electrophoresis (PAGE). To explore the basic principles of wire formation, we studied the effects of base sequence, method of preparation, temperature, and oligonucleotide concentration. Both DLS and PAGE show that thermal annealing induces much less macromolecular self-assembly than dialysis. The degree of assembly and consequently length of G-wires (5-6 nm) are well resolved by both methods for DNA sequences with intermediate length, while some discrepancies appear for the shortest and longest sequences. As expected, the longest DNA sequence gives the longest macromolecular aggregates with a length of about 11 nm as estimated by DLS. The quadruplex topologies show no concentration dependence in the investigated DNA concentration range (0.1 mM–0.4 mM) and no structural change upon heating. DNA dynamic light scattering DLS polyacrylamide gel electrophoresis PAGE genetics DNK dinamično sipanje svetlobe DLS poliakrilamidna gelska elektroforeza PAGE genetika true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-06-14 13:21:28 2017-06-14 13:22:33 2022-07-10 03:06:51 2021-05-15 03:33:59 2010 0 0 str. 1-8 Letn. 2010 2010 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2090-021X 53 14756118 23737127 10.4061/2010/431651 2090-021X URN:SI:UM:DK:TBKSDBHN Journal_of_Nucleic_Acids_2010_Spindler_et_al._Effect_of_Base_Sequence_on_G-Wire_Formation_in_Solution.pdf Journal_of_Nucleic_Acids_2010_Spindler_et_al._Effect_of_Base_Sequence_on_G-Wire_Formation_in_Solution.pdf 1 82E99D3677ABF3EDAAAC176691B19100 b3b286c7fa2aca3f7de24bf1a2648168419defb0fe2d06b13511a6a699a26e8d 3ea3278c-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=112539 http://www.hindawi.com/journals/jna/2010/431651/ 1 https://dk.um.si/Dokument.php?lang=slv&id=112538 Fakulteta za strojništvo 0 0 0