20.500.12556/DKUM-66202 Guanosine quadruplexes in solution a small-angle X-ray scattering analysis of temperature effects on self-assembling of deoxyguanosine monophosphate We investigated quadruplex formation in aqueous solutions of -deoxyriboguanosine -monophosphate, d(pG), which takes place in the absence of the covalent axial backbone. A series of in-solution small angle X-ray scattering experiments on d(pG) have been performed as a function of temperature in the absence of excess salt, at a concentration just above the critical one at which self-assembling occurs. A global fit approach has been used to derive composition and size distribution of the scattering particles as a function of temperature. The obtained results give thermodynamical justification for the observed phase-behavior, indicating that octamer formation is essential for quadruplex elongation. Our investigation shows that d(pG) quadruplexes are very suitable to assess the potential of G-quadruplex formation and to study the self-assembling thermodynamics. G-quadruplexes guanosine G-kvadrupleksi gvanozin true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-06-14 13:47:06 2017-06-14 13:48:21 2022-07-10 03:06:51 2021-05-15 03:34:01 2010 0 0 str. 1-10 Letn. 2010 2010 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2090-021X 53 14756118 23736871 10.4061/2010/472478 2090-021X URN:SI:UM:DK:53AFWAE0 Journal_of_Nucleic_Acids_2010_Mariani_et_al._Guanosine_Quadruplexes_in_Solution_A_Small-Angle_X-Ray_Scattering_Analysis_of_Temperature_E.pdf Journal_of_Nucleic_Acids_2010_Mariani_et_al._Guanosine_Quadruplexes_in_Solution_A_Small-Angle_X-Ray_Scattering_Analysis_of_Temperature_E.pdf 1 AAFB1759E10519F5E2533BEE59018657 b5f769e4b1ae25a926c6453e3739810af54d7c167b8ef8bef16e13bcec823dd7 3eb16b87-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=112543 http://www.hindawi.com/journals/jna/2010/472478/ 1 https://dk.um.si/Dokument.php?lang=slv&id=112542 Fakulteta za strojništvo 0 0 0