20.500.12556/DKUM-66288 Platelet-rich plasma, especially when combined with a TGF-ß inhibitor promotes proliferation, viability and myogenic differentiation of myoblasts in vitro Regeneration of skeletal muscle after injury is limited by scar formation, slow healing time and a high recurrence rate. A therapy based on platelet-rich plasma (PRP) has become a promising lead for tendon and ligament injuries in recent years, however concerns have been raised that PRP-derived TGF-β could contribute to fibrotic remodelling in skeletal muscle after injury. Due to the lack of scientific grounds for a PRP -based muscle regeneration therapy, we have designed a study using human myogenic progenitors and evaluated the potential of PRP alone and in combination with decorin (a TGF-β inhibitor), to alter myoblast proliferation, metabolic activity, cytokine profile and expression of myogenic regulatory factors (MRFs). Advanced imaging multicolor single-cell analysis enabled us to create a valuable picture on the ratio of quiescent, activated and terminally committed myoblasts in treated versus control cell populations. Finally high-resolution confocal microscopy validated the potential of PRP and decorin to stimulate the formation of polynucleated myotubules. PRP was shown to down-regulate fibrotic cytokines, increase cell viability and proliferation, enhance the expression of MRFs, and contribute to a significant myogenic shift during differentiation. When combined with decorin further synergistc effects were identified. These results suggest that PRP could not only prevent fibrosis but could also stimulate muscle commitment, especially when combined with a TGF-β inhibitor. muscles skeletal injuries TGF-beta plasma thrombocytes myoblasts fibrosis prevention regeneration mišice skeletne poškodbe TGF-beta plazma bogata s trombociti mioblasti fibroza preprečevanje regeneracija true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-06-19 11:52:20 2017-06-19 11:53:06 2022-07-11 03:06:04 2019-07-11 15:42:27 2015 0 0 str. 1-13 št. 2 Letn. 10 13. feb. 2015 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1932-6203 616-001 2005896 5269311 10.1371/journal.pone.0117302 1932-6203 URN:SI:UM:DK:J1A0HJD7 PLOS_ONE_2015_Kelc_et_al._Platelet-Rich_Plasma,_Especially_When_Combined_with_a_TGF-β_Inhibitor_Promotes_Proliferation,_Viability_and_My.PDF PLOS_ONE_2015_Kelc_et_al._Platelet-Rich_Plasma,_Especially_When_Combined_with_a_TGF-β_Inhibitor_Promotes_Proliferation,_Viability_and_My.PDF 1 803D5040EBB2FC648FF63974EC178847 0c407a68c32c7e82b8893fdfab6d146468adbf1592c31f0e3d056d04e4e7203e 43656bb1-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=112762 http://dx.plos.org/10.1371/journal.pone.0117302 1 https://dk.um.si/Dokument.php?lang=slv&id=112761 Medicinska fakulteta 0 0 0