20.500.12556/DKUM-66298 Size-dependent effects of gold nanoparticles uptake on maturation and antitumor functions of human dendritic cells in vitro Gold nanoparticles (GNPs) are claimed as outstanding biomedical tools for cancer diagnostics and photo-thermal therapy, but without enough evidence on their potentially adverse immunological effects. Using a model of human dendritic cells (DCs), we showed that 10 nm- and 50 nm-sized GNPs (GNP10 and GNP50, respectively) were internalized predominantly via dynamin-dependent mechanisms, and they both impaired LPS-induced maturation and allostimulatory capacity of DCs, although the effect of GNP10 was more prominent. However, GNP10 inhibited LPS-induced production of IL-12p70 by DCs, and potentiated their Th2 polarization capacity, while GNP50 promoted Th17 polarization. Such effects of GNP10 correlated with a stronger inhibition of LPS-induced changes in Ca2+ oscillations, their higher number per DC, and more frequent extraendosomal localization, as judged by live-cell imaging, proton, and electron microscopy, respectively. Even when released from heat-killed necrotic HEp-2 cells, GNP10 inhibited the necrotic tumor cell-induced maturation and functions of DCs, potentiated their Th2/Th17 polarization capacity, and thus, impaired the DCs% capacity to induce T cell-mediated anti-tumor cytotoxicity in vitro. Therefore, GNP10 could potentially induce more adverse DC-mediated immunological effects, compared to GNP50. gold nanoparticles antitumor function zlato nanodelci protitumorna funkcija true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-06-19 13:07:21 2017-06-19 13:08:04 2022-07-11 03:06:07 2019-07-11 15:42:45 2014 0 0 str. št. 13 Letn. 9 2014 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1932-6203 577 2005896 27671847 10.1371/journal.pone.0096584 1932-6203 URN:SI:UM:DK:KANVP8VJ PLoS_ONE_2014_Tomic_et_al._Size-Dependent_Effects_of_Gold_Nanoparticles_Uptake_on_Maturation_and_Antitumor_Functions_of_Human_Dendritic.PDF PLoS_ONE_2014_Tomic_et_al._Size-Dependent_Effects_of_Gold_Nanoparticles_Uptake_on_Maturation_and_Antitumor_Functions_of_Human_Dendritic.PDF 1 039D261D5C104C75F92F9DE5F44BDCA5 585d05b78701ae4bf4189ddc5014d0dbb70706552f3635e27dfff64b4ede6023 43cd562e-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=112782 http://dx.plos.org/10.1371/journal.pone.0096584 1 https://dk.um.si/Dokument.php?lang=slv&id=112781 Fakulteta za strojništvo 0 0 0