20.500.12556/DKUM-66303 The relationship between membrane potential and calcium dynamics in glucose-stimulated beta cell syncytium in acute mouse pancreas tissue slices Oscillatory electrical activity is regarded as a hallmark of the pancreatic beta cell glucose-dependent excitability pattern. Electrophysiologically recorded membrane potential oscillations in beta cells are associated with in-phase oscillatory cytosolic calcium activity ([Ca2+]i) measured with fluorescent probes. Recent high spatial and temporal resolution confocal imaging revealed that glucose stimulation of beta cells in intact islets within acute tissue slices produces a [Ca2+]i change with initial transient phase followed by a plateau phase with highly synchronized [Ca2+]i oscillations. Here, we aimed to correlate the plateau [Ca2+]i oscillations with the oscillations of membrane potential using patch-clamp and for the first time high resolution voltage-sensitive dye based confocal imaging. Our results demonstrated that the glucose-evoked membrane potential oscillations spread over the islet in a wave-like manner, their durations and wave velocities being comparable to the ones for [Ca2+]i oscillations and waves. High temporal resolution simultaneous records of membrane potential and [Ca2+]i confirmed tight but nevertheless limited coupling of the two processes, with membrane depolarization preceding the [Ca2+]i increase. The potassium channel blocker tetraethylammonium increased the velocity at which oscillations advanced over the islet by several-fold while, at the same time, emphasized differences in kinetics of the membrane potential and the [Ca2+]i. The combination of both imaging techniques provides a powerful tool that will help us attain deeper knowledge of the beta cell network. glucose pancreas mice glukoza trebušna slinavka miši true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-06-19 13:31:19 2017-06-19 13:32:13 2022-07-11 03:06:09 2019-07-11 15:42:55 2013 0 0 str. 1-16 št. 12 Letn. 8 2013 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1932-6203 612.349.7 2005896 512362552 10.1371/journal.pone.0082374 1932-6203 URN:SI:UM:DK:H6SGPS3R PLoS_ONE_2013_Dolensek_et_al._The_Relationship_between_Membrane_Potential_and_Calcium_Dynamics_in_Glucose-Stimulated_Beta_Cell_Syncytium.PDF PLoS_ONE_2013_Dolensek_et_al._The_Relationship_between_Membrane_Potential_and_Calcium_Dynamics_in_Glucose-Stimulated_Beta_Cell_Syncytium.PDF 1 8BEB97CED03AC58BDFE7F24E9DE6BF0F 23bea89e1dc53f7da8a758f4a59805287df63dea9d63e84fbb81435bd1c03402 43f8416c-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=112789 http://dx.plos.org/10.1371/journal.pone.0082374 1 https://dk.um.si/Dokument.php?lang=slv&id=112788 Medicinska fakulteta 0 0 0