20.500.12556/DKUM-66304 The SOS response master regulator LexA is associated with sporulation, motility and biofilm formation in Clostridium difficile The LexA regulated SOS network is a bacterial response to DNA damage of metabolic or environmental origin. In Clostridium difficile, a nosocomial pathogen causing a range of intestinal diseases, the in-silico deduced LexA network included the core SOS genes involved in the DNA repair and genes involved in various other biological functions that vary among different ribotypes. Here we describe the construction and characterization of a lexA ClosTron mutant in C. difficile R20291 strain. The mutation of lexA caused inhibition of cell division resulting in a filamentous phenotype. The lexA mutant also showed decreased sporulation, a reduction in swimming motility, greater sensitivity to metronidazole, and increased biofilm formation. Changes in the regulation of toxin A, but not toxin B, were observed in the lexA mutant in the presence of sub-inhibitory concentrations of levofloxacin. C. difficile LexA is, therefore, not only a regulator of DNA damage but also controls many biological functions associated with virulence. toxins bacterial sporulation DNA damage antibiotics toksini bakterijska sporulacija poškodba DNK antibiotiki true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-06-19 13:36:52 2017-06-19 13:37:46 2022-07-11 03:06:10 2019-07-11 15:42:57 2015 0 0 str. 1-17 št. 12 Letn. 10 2015 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1932-6203 577.21 2005896 3703631 10.1371/journal.pone.0144763 1932-6203 URN:SI:UM:DK:PGUSMRAW PLOS_ONE_2015_Walter_et_al._The_SOS_Response_Master_Regulator_LexA_Is_Associated_with_Sporulation,_Motility_and_Biofilm_Formation_in_Clo.PDF PLOS_ONE_2015_Walter_et_al._The_SOS_Response_Master_Regulator_LexA_Is_Associated_with_Sporulation,_Motility_and_Biofilm_Formation_in_Clo.PDF 1 8212803A5B5BB5B9F73B627CB0B9F4A4 1166967020320878f31a6c1464d32220b63a97311de7a33c424d6eb6ad426f77 4414df18-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=112792 http://dx.plos.org/10.1371/journal.pone.0144763 1 https://dk.um.si/Dokument.php?lang=slv&id=112791 Medicinska fakulteta 0 0 0