20.500.12556/DKUM-66355 Protein adsorption on various plasma-treated polyethylene-terephthalate substrates Protein adhesion and cell response to plasma-treated polymer surfaces were studied. The polymer polyethylene terephthalate (PET) was treated in either an oxygen plasma to make the surface hydrophilic, or a tetrafluoromethane CF4 plasma to make the surface hydrophobic. The plasma source was radiofrequency (RF) discharge. The adsorption of albumin and other proteins from a cell-culture medium onto these surfaces was studied using a quartz crystal microbalance (QCM), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The cellular response to plasma-treated surfaces was studied as well using an MTT assay and scanning electron microscopy (SEM). The fastest adsorption rate was found on the hydrophilic oxygen plasma-treated sample, and the lowest was found on the pristine untreated sample. Additionally, the amount of adsorbed proteins was higher for the oxygen-plasma-treated surface, and the adsorbed layer was more viscoelastic. In addition, cell adhesion studies support this finding because the best cell adhesion was observed on oxygen-plasma-treated substrates. oxygen and fluorine plasma treatment polymer surface modification protein adsorption cell adhesion quartz crystal microbalance QCM kisik fluor plazma površinska modifikacija polimera adsorpcija proteinov adhezija celic kvarčna mikrotehtnica QCM true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-06-22 08:50:17 2017-06-22 08:51:16 2022-07-11 03:06:27 2019-07-11 15:44:37 2013 0 0 str. 12441-12463 št. 10 Letn. 18 2013 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1420-3049 533 18462981 27136551 10.3390/molecules181012441 1420-3049 URN:SI:UM:DK:QQ5MC8JC Molecules_2013_Recek_et_al._Protein_Adsorption_on_Various_Plasma-Treated_Polyethylene_Terephthalate_Substrates.pdf Molecules_2013_Recek_et_al._Protein_Adsorption_on_Various_Plasma-Treated_Polyethylene_Terephthalate_Substrates.pdf 1 EB2F9C62655A876BA5973F3DACBD291E 682f8b94c04f7069c97f991039397c4c1fd67c8266fabea57850ee6a9c45bef6 4771a867-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=112911 http://www.mdpi.com/1420-3049/18/10/12441/ 1 https://dk.um.si/Dokument.php?lang=slv&id=112910 Fakulteta za strojništvo 0 0 0