20.500.12556/DKUM-66358 Effect of Bifidobacterium breve on the intestinal microbiota of coeliac children on gluten free diet a pilot study Coeliac disease (CD) is associated with alterations of the intestinal microbiota. Although several Bifidobacterium strains showed anti-inflammatory activity and prevention of toxic gliadin peptides generation in vitro, few data are available on their efficacy when administered to CD subjects. This study evaluated the effect of administration for three months of a food supplement based on two Bifidobacterium breve strains (B632 and BR03) to restore the gut microbial balance in coeliac children on a gluten free diet (GFD). Microbial DNA was extracted from faeces of 40 coeliac children before and after probiotic or placebo administration and 16 healthy children (Control group). Sequencing of the amplified V3-V4 hypervariable region of 16S rRNA gene as well as qPCR of Bidobacterium spp., Lactobacillus spp., Bacteroides fragilis group Clostridium sensu stricto and enterobacteria were performed. The comparison between CD subjects and Control group revealed an alteration in the intestinal microbial composition of coeliacs mainly characterized by a reduction of the Firmicutes/Bacteroidetes ratio, of Actinobacteria and Euryarchaeota. Regarding the effects of the probiotic, an increase of Actinobacteria was found as well as a re-establishment of the physiological Firmicutes/Bacteroidetes ratio. Therefore, a three-month administration of B. breve strains helps in restoring the healthy percentage of main microbial components. coeliac disease gluten free diet probiotic Bifidobacterium breve intestinal microbiota qPCR next generation sequencing celiakija brezglutenska dieta probiotik Bifidobacterium breve črevesna mikrobiota qPCR verižna reakcija s polimerazo sekvenciranje nove generacije NGS true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-06-22 09:13:12 2017-06-22 09:14:33 2022-07-11 03:06:28 2019-07-11 15:44:44 2016 0 0 str. 1-16 št. 10 Letn. 8 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2072-6643 579 2948140 4240940 10.3390/nu8100660 2072-6643 URN:SI:UM:DK:XBMSTWKM Nutrients_2016_Quagliariello_et_al._Effect_of_Bifidobacterium_breve_on_the_Intestinal_Microbiota_of_Coeliac_Children_on_a_Gluten_Free_Di.pdf Nutrients_2016_Quagliariello_et_al._Effect_of_Bifidobacterium_breve_on_the_Intestinal_Microbiota_of_Coeliac_Children_on_a_Gluten_Free_Di.pdf 1 0F525EBD0B3F155D50F2F79C97A055AA 01451bdbf3faa79890b5e16f639d03feea2a79f6b70dd83344124b60b765f004 47a36c07-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=112917 http://www.mdpi.com/2072-6643/8/10/660 1 https://dk.um.si/Dokument.php?lang=slv&id=112916 Fakulteta za kmetijstvo in biosistemske vede 0 0 0