20.500.12556/DKUM-66405 Native cellulose nanofibrills induce immune tolerance in vitro by acting on dendritic cells Cellulose nanofibrills (CNFs) are attractive biocompatible, natural nanomaterials for wide biomedical applications. However, the immunological mechanisms of CNFs have been poorly investigated. Considering that dendritic cells (DCs) are the key immune regulatory cells in response to nanomaterials, our aim was to investigate the immunological mechanisms of CNFs in a model of DC-mediated immune response. We found that non-toxic concentrations of CNFs impaired the differentiation, and subsequent maturation of human monocyte-derived (mo)-DCs. In a co-culture with CD4+T cells, CNF-treated mo-DCs possessed a weaker allostimulatory and T helper (Th)1 and Th17 polarizing capacity, but a stronger capacity to induce Th2 cells and CD4+CD25hiFoxP3hi regulatory T cells. This correlated with an increased immunoglobulin-like transcript-4 and indolamine dioxygenase-1 expression by CNF-treated mo-DCs, following the partial internalization of CNFs and the accumulation of CD209 and actin bundles at the place of contacts with CNFs. Cumulatively, we showed that CNFs are able to induce an active immune tolerance by inducing tolerogenic DCs, which could be beneficial for the application of CNFs in wound healing and chronic inflammation therapies. nanocellulose cytotoxicity T-cell proliferation cytokine production in vitro nanoceluloze citotoksičnost produkcija citokinov in vitro true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-06-23 12:03:46 2017-06-23 12:05:40 2022-07-11 03:06:46 2019-07-11 15:40:46 2016 0 0 str. 1-14 Letn. 6 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2045-2322 576/577 18727432 19888662 10.1038/srep31618 2045-2322 URN:SI:UM:DK:OILL9FWG Scientific_Reports_2016_Tomic_et_al._Native_cellulose_nanofibrills_induce_immune_tolerance_in_vitro_by_acting_on_dendritic_cells.pdf Scientific_Reports_2016_Tomic_et_al._Native_cellulose_nanofibrills_induce_immune_tolerance_in_vitro_by_acting_on_dendritic_cells.pdf 1 5DDD71153D986D2C1B90AD9686E6D5B0 71203ccdd54271ff9e9286663eb93b9e9c781569e6f479b9d7ad928ee917d322 4ac52614-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=113031 http://www.nature.com/articles/srep31618 1 https://dk.um.si/Dokument.php?lang=slv&id=113030 Fakulteta za strojništvo 0 0 0