20.500.12556/DKUM-66408 Polyester type polyHIPE scaffolds with an interconnected porous structure for cartilage regeneration Development of artificial materials for the facilitation of cartilage regeneration remains an important challenge in orthopedic practice. Our study investigates the potential for neocartilage formation within a synthetic polyester scaffold based on the polymerization of high internal phase emulsions. The fabrication of polyHIPE polymer (PHP) was specifically tailored to produce a highly porous (85%) structure with the primary pore size in the range of 50–170 μm for cartilage tissue engineering. The resulting PHP scaffold was proven biocompatible with human articular chondrocytes and viable cells were observed within the materials as evaluated using the Live/Dead assay and histological analysis. Chondrocytes with round nuclei were organized into multicellular layers on the PHP surface and were observed to grow approximately 300 μm into the scaffold interior. The accumulation of collagen type 2 was detected using immunohistochemistry and chondrogenic specific genes were expressed with favorable collagen type 2 to 1 ratio. In addition, PHP samples are biodegradable and their baseline mechanical properties are similar to those of native cartilage, which enhance chondrocyte cell growth and proliferation. polyester polymerization polyHIPE poliestri polyHIPE polimerizacija true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-06-23 12:18:48 2017-06-23 12:20:04 2022-07-11 03:06:47 2019-07-11 15:46:25 2016 0 0 str. 1-11 Letn. 6 Published online: 24 June 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2045-2322 66 18727432 19662102 10.1038/srep28695 2045-2322 URN:SI:UM:DK:7WJGSGNF Scientific_Reports_2016_Naranda_et_al._Polyester_type_polyHIPE_scaffolds_with_an_interconnected_porous_structure_for_cartilage_regenerat.pdf Scientific_Reports_2016_Naranda_et_al._Polyester_type_polyHIPE_scaffolds_with_an_interconnected_porous_structure_for_cartilage_regenerat.pdf 1 A4B815E5AF0F76A50B6035FAB24FE2A6 d576517b044e6063b5ec669f1f7e0dc693cce6b6a07ca8dbf59003bef24e507e 4aefa5b4-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=113037 http://www.nature.com/articles/srep28695 1 https://dk.um.si/Dokument.php?lang=slv&id=113036 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0