20.500.12556/DKUM-66508 Typing Clostridium difficile strains based on tandem repeat sequences Background: Genotyping of epidemic Clostridium difficile strains is necessary to track their emergence and spread. Portability of genotyping data is desirable to facilitate inter-laboratory comparisons and epidemiological studies. Results: This report presents results from a systematic screen for variation in repetitive DNA in the genome of C. difficile. We describe two tandem repeat loci, designated 'TR6' and 'TR10', which display extensive sequence variation that may be useful for sequence-based strain typing. Based on an investigation of 154 C. difficile isolates comprising 75 ribotypes, tandem repeat sequencing demonstrated excellent concordance with widely used PCR ribotyping and equal discriminatory power. Moreover, tandem repeat sequences enabled the reconstruction of the isolates' largely clonal population structure and evolutionary history. Conclusion: We conclude that sequence analysis of the two repetitive loci introduced here may be highly useful for routine typing of C. difficile. Tandem repeat sequence typing resolves phylogenetic diversity to a level equivalent to PCR ribotypes. DNA sequences may be stored in databases accessible over the internet, obviating the need for the exchange of reference strains. Clostridium difficile microbiology strain typing Clostridium difficile mikrobiologija tipizacija sevov true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-06-29 09:21:23 2017-06-29 09:22:14 2022-07-11 03:07:23 2019-07-11 15:49:51 2009 0 0 str. 1-11 Letn. 9 2009 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1471-2180 579.6 2441492 652063 10.1186/1471-2180-9-6 1471-2180 URN:SI:UM:DK:DFSXNGHG BMC_Microbiology_2009_ZaiSS_et_al._Typing_Clostridium_difficile_strains_based_on_tandem_repeat_sequences.pdf BMC_Microbiology_2009_ZaiSS_et_al._Typing_Clostridium_difficile_strains_based_on_tandem_repeat_sequences.pdf 1 7935102399729F22747BA7836244BE54 1e0d58737a815486a14010095ce7184cd4a4753b4a5f4b28eed82bd31ee82bba 51bf6021-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=113315 http://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-9-6 1 https://dk.um.si/Dokument.php?lang=slv&id=113314 Medicinska fakulteta 0 0 0