20.500.12556/DKUM-66509 TMPRSS2:ERG gene aberrations may provide insight into pT stage in prostate cancer Background: TMPRSS2:ERG gene aberration may be a novel marker that improves risk stratification of prostate cancer before definitive cancer therapy, but studies have been inconclusive. Methods: The study cohort consisted of 202 operable prostate cancer Slovenian patients who underwent laparoscopic radical prostatectomy. We retrospectively constructed tissue microarrays of their prostatic specimens for fluorescence in situ hybridization, with appropriate signals obtained in 148 patients for subsequent statistical analyses. Results: The following genetic aberrations were found: TMPRSS2:ERG fusion, TMPRSS2 split (a non-ERG translocation) and ERG split (an ERG translocation without involvement of TMPRSS2). TMPRSS2:ERG gene fusion happened in 63 patients (42 %), TMPRSS2 split in 12 patients and ERG split in 8 patients. Association was tested between TMPRSS2:ERG gene fusion and several clinicopathological variables, i.e., pT stage, extended lymph node dissection status, and Gleason score, correcting for multiple comparisons. Only the association with pT stage was significant at p = 0.05: Of 62 patients with pT3 stage, 34 (55 %) had TMPRSS2:ERG gene fusion. In pT3 stage patients, stronger (but not significant) association between eLND status and TMPRSS2:ERG gene fusion was detected. We detected TMPRSS2:ERG gene fusion in 64 % of the pT3 stage patients where we did not perform an extended lymph node dissection. Conclusions: Our results indicate that it is possible to predict pT3 stage at final histology from TMPRSS2:ERG gene fusion at initial core needle biopsy. FISH determination of TMPRSS2:ERG gene fusion may be particularly useful for patients scheduled to undergo a radical prostatectomy in order to improve oncological and functional results. FISH predicting pT stage radical prostatectomy prostate cancer TMPRSS2:ERG fusion prostata rak (medicina) radikalna prostatektomija geni aberacija TMPRSS2:ERG fuzija fluorescentna in situ hibridizacija true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-06-29 09:26:31 2017-06-29 09:27:39 2022-07-11 03:07:23 2019-07-11 15:49:53 2016 0 0 str. 1-6 Letn. 16 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1471-2490 616.65-006 2447380 29626407 10.1186/s12894-016-0160-8 1471-2490 URN:SI:UM:DK:GBWSL9IB BMC_Urology_2016_Krstanoski_et_al._TMPRSS2ERG_gene_aberrations_may_provide_insight_into_pT_stage_in_prostate_cancer.pdf BMC_Urology_2016_Krstanoski_et_al._TMPRSS2ERG_gene_aberrations_may_provide_insight_into_pT_stage_in_prostate_cancer.pdf 1 E38AD6B2C0AF4970A692CDF8EF508631 c7e2bcc4b518bc78214f2712b9ae59d566542e6f0adcfca28e11c83cdf0c69a4 51cdd61b-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=113317 http://bmcurol.biomedcentral.com/articles/10.1186/s12894-016-0160-8 1 https://dk.um.si/Dokument.php?lang=slv&id=113316 Medicinska fakulteta 0 0 0