20.500.12556/DKUM-66519 An evaluation of SOX2 and hTERC gene amplifications as screening markers in oral and oropharyngeal squamous cell carcinomas Background: Oral and oropharyngeal squamous cell carcinomas (OSCC) are among the most common cancers. The poor survival rate among oral cancer patients can be attributed to several factors, one of them being lack of early detection. A key approach to this problem would be to detect potentially malignant lesion at their early stage. Using the FISH technique, oral brush cytology slides can be an easy and rapid screening approach for malignant cell detection. The present study was designed to detect hTERC and SOX2 amplifications in OSSC exfoliative tumor cells and evaluate whether those two gene amplifications might serve as a supportive biomarker in early detection and diagnosis of oral and oropharyngeal SCC. Results: Brush biopsies were collected from exophytic and exulcerated oral and oropharyngeal lesions of the oral cavity of 71 patients and 22 healthy controls. FISH techniques using a TERC-specific DNA probe and a SOX2 DNA specific probe both combined with a centromere 3-specific control probe was performed on the cytology slides. A 100 squamous epithelial cell nuclei of the smears per slide were analysed. As abnormal FISH pattern were considered amplified and polyploid patterns. From 71 brush biopsies of oropharynx and other locations in oral cavity analysed by FISH 49 were considered to be abnormal (69%). The over representation of polyploidy and/or TERC/SOX2 amplification in tumour samples was statistically significant when compared to controls (p = 0.01). Conclusion: SOX2 and TERC gene amplifications are common in all squamous cell carcinomas and their detection in early stages could be crucial for early detection and more accurate prognosis. Our study strongly suggests that early detection by FISH on cytobrushed samples could be a possible non-invasive screening method even before a tissue biopsy is performed. SOX and hTERC gene amplifications brush biopsy Oral oropharyngeal squamous cell carcinoma gen amplifikacija orofaringealni ploščatocelični karcinom diagnostika tumorske celice brisi rak zgodnje odkrivanje In situ hibridizacija fluorescenčna medicinska genetika true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-06-29 12:50:30 2017-06-29 12:51:38 2022-07-11 03:07:27 2019-07-11 15:50:11 0 0 0 str. 1-8 Letn. 7 Jan. 2014 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1755-8166 616.31-006:575 3085332 4897599 10.1186/1755-8166-7-5 1755-8166 URN:SI:UM:DK:VSEGDVBD Molecular_Cytogenetics_2014_Kokalj_Vokac_et_al._An_evaluation_of_SOX2_and_hTERC_gene_amplifications_as_screening_markers_in_oral_and_oro.pdf Molecular_Cytogenetics_2014_Kokalj_Vokac_et_al._An_evaluation_of_SOX2_and_hTERC_gene_amplifications_as_screening_markers_in_oral_and_oro.pdf 1 4419DD03C17F26DAD7F1237251E94D53 5a66920334c66cf6c770f5559a0c1c8b9297877f61f8ce63a0ae5e153042ed00 52b78e98-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=113348 http://molecularcytogenetics.biomedcentral.com/articles/10.1186/1755-8166-7-5 1 https://dk.um.si/Dokument.php?lang=slv&id=113347 Medicinska fakulteta 0 0 0