20.500.12556/DKUM-66522 Identification of risk factors influencing Clostridium difficile prevalence in middle-size dairy farms Farm animals have been suggested to play an important role in the epidemiology of Clostridium difficile infection (CDI) in the community. The purpose of this study was to evaluate risk factors associated with C. difficile dissemination in family dairy farms, which are the most common farming model in the European Union. Environmental samples and fecal samples from cows and calves were collected repeatedly over a 1 year period on 20 mid-size family dairy farms. Clostridium difficile was detected in cattle feces on all farms using qPCR. The average prevalence between farms was 10% (0-44.4%) and 35.7% (3.7-66.7%) in cows and calves, respectively. Bacterial culture yielded 103 C. difficile isolates from cattle and 61 from the environment. Most C. difficile isolates were PCR-ribotype 033. A univariate mixed effect model analysis of risk factors associated dietary changes with increasing C. difficile prevalence in cows (P = 0.0004); and dietary changes (P = 0.004), breeding Simmental cattle (P = 0.001), mastitis (P = 0.003) and antibiotic treatment (P = 0.003) in calves. Multivariate analysis of risk factors found that dietary changes in cows (P = 0.0001) and calves (P = 0.002) increase C. difficile prevalence; mastitis was identified as a risk factor in calves (P = 0.001). This study shows that C. difficile is common on dairy farms and that shedding is more influenced by farm management than environmental factors. Based on molecular typing of C. difficile isolates, it could also be concluded that family dairy farms are currently not contributing to increased CDI incidence. farm animals clostridium difficile infection (CDI) dairy farms domače živali difficile clostridium okužba (CDI) kmetije za proizvodnjo mleka true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-06-29 13:16:32 2017-06-29 13:17:45 2022-07-11 03:07:29 2019-07-11 15:50:17 2016 0 0 str. 1-11 Letn. 47 Mar. 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1297-9716 636.09 3013512 32539609 10.1186/s13567-016-0326-0 1297-9716 URN:SI:UM:DK:QOG1TUZT Veterinary_Research_2016_Bandelj_et_al._Identification_of_risk_factors_influencing_Clostridium_difficile_prevalence_in_middle-size_dairy.pdf Veterinary_Research_2016_Bandelj_et_al._Identification_of_risk_factors_influencing_Clostridium_difficile_prevalence_in_middle-size_dairy.pdf 1 74D8C01AD1C21F9B76775ABD5D2895D9 9565abaff3e3e91073827dcaa9b549c4b7b5f0d54f1cfc583a0283deae7470a7 52e26fdd-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=113354 http://www.veterinaryresearch.org/content/47/1/41 1 https://dk.um.si/Dokument.php?lang=slv&id=113353 Medicinska fakulteta 0 0 0