20.500.12556/DKUM-66788 Use of non-conventional cell disruption method for extraction of proteins from black yeasts The influence of pressure and treatment time on cells disruption of different black yeasts and on activities of extracted proteins using supercritical carbon dioxide process was studied. The cells of three different black yeasts Phaeotheca triangularis, Trimatostroma salinum, and Wallemia ichthyophaga were exposed to supercritical carbon dioxide (SC $CO_2$) by varying pressure at fixed temperature (35°C). The black yeasts cell walls were disrupted, and the content of the cells was spilled into the liquid medium. The impact of SC $CO_2$ conditions on secretion of enzymes and proteins from black yeast cells suspension was studied. The residual activity of the enzymes cellulase, $beta$-glucosidase, $alpha$-amylase, and protease was studied by enzymatic assay. The viability of black yeast cells was determined by measuring the optical density of the cell suspension at 600 nm. The total protein concentration in the suspension was determined on UV–Vis spectrophotometer at 595 nm. The release of intracellular and extracellular products from black yeast cells was achieved. Also, the observation by an environmental scanning electron microscopy shows major morphological changes with SC $CO_2$-treated cells. The advantages of the proposed method are in a simple use, which is also possible for heat-sensitive materials on one hand and on the other hand integration of the extraction of enzymes and their use in biocatalytical reactions. P. triangularis W. ichtyophaga T. salinum supercritical carbon dioxide enzyme activity cells P. triangularis W. ichtyophaga T. salinum superkritični ogljikov dioksid aktivnost encimov celice true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-07-10 12:48:50 2017-07-10 12:49:39 2022-08-01 13:10:17 2019-07-11 15:58:57 2016 0 0 str. 1-12 Letn. 4 April 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2296-4185 66 523093017 19560726 10.3389/fbioe.2016.00033 2296-4185 URN:SI:UM:DK:6BTSLNMZ Frontiers_in_Bioengineering_and_Biotechnology_2016_Colnik_et_al._Use_of_Non-Conventional_Cell_Disruption_Method_for_Extraction_of_Protei.pdf Frontiers_in_Bioengineering_and_Biotechnology_2016_Colnik_et_al._Use_of_Non-Conventional_Cell_Disruption_Method_for_Extraction_of_Protei.pdf 1 4C3C9BE60F6C16F194250FD079612AD4 ea8024b2c0f3b4fc23aaadb3f15cd5a7d60d915f635e70ad2d25a8396fb1ca2b 63e3d209-7c0e-11eb-bb7a-00155d0001ca 20.500.12556/dkum/f22c92d9-01b8-4787-9a70-67f64f61e592 https://dk.um.si/Dokument.php?lang=slv&id=113964 http://journal.frontiersin.org/article/10.3389/fbioe.2016.00033/full 1 https://dk.um.si/Dokument.php?lang=slv&id=113963 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0