20.500.12556/DKUM-66997 16S rRNA in situ hybridization followed by flow cytometry for rapid identification of acetic acid bacteria involved in submerged industrial vinegar production Acetic acid bacteria are involved in many biotechnological processes such as vitamin C, gluconic acid, miglitol or acetic acid production, and others. For a technologist trying to control the industrial process, the ability to follow the microbiological development of the process is thus of importance. During the past few years hybridization in a combination with flow cytometry has oft en been used for this purpose. Since vinegar is a liquid, it is an ideal matrix for flow cytometry analysis. In this work we have constructed a specific probe for highly acetic acid-resistant species of the acetic acid bacteria and a protocol for in situ hybridization, which in combination with flow cytometry enables direct monitoring of bacteria producing vinegar with >10 % of acetic acid. The approach was successfully applied for monitoring microbiota during industrial vinegar production. microbiology acetic acid bacteria flow cytometry mikrobiologija ocetnokislinske bakterije pretočna citometrija true true true University of Zagreb Faculty of Food Technology and Biotechnology Angleški jezik Slovenski jezik Znanstveno delo 2017-07-24 11:58:08 2017-07-24 11:59:43 2026-09-10 14:02:52 2019-07-11 16:05:53 2016 0 0 4 str.. št. 1 Letn. 54 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2014-12-20 2015-10-13 0000-00-00 1330-9862 579.66 40116737 22612232 10.17113/ftb.54.01.16.4041 1330-9862 URN:SI:UM:DK:QWDOJMCU Food_Technology_and_Biotechnology_2016_Trcek,_Lipoglavsek,_Avgustin_16S_rRNA_in_situ_Hybridization_Followed_by_Flow_Cytometry_for_Rapid.pdf Food_Technology_and_Biotechnology_2016_Trcek,_Lipoglavsek,_Avgustin_16S_rRNA_in_situ_Hybridization_Followed_by_Flow_Cytometry_for_Rapid.pdf 1 7809705424A5B6A20D971CB67CD2D3E2 59a543c5becc71d6923c7502add2fd876188d44890346bf16ddc7ccd00c67b21 7249d1b9-7c0e-11eb-bb7a-00155d0001ca 20.500.12556/dkum/7d57fe7f-ea23-45d0-9276-d7210ee370c2 https://dk.um.si/Dokument.php?lang=slv&id=114500 http://www.ftb.com.hr/index.php/archives/156-volume-54-issue-no-1/1404-16s-rrna-in-situ-hybridization-followed-by-flow-cytometry-for-rapid-identifi-cation-of-acetic-acid-bacteria-involved-in-submerged-industrial-vinegar-production 1 https://dk.um.si/Dokument.php?lang=slv&id=114499 Fakulteta za naravoslovje in matematiko 0 0 0