20.500.12556/DKUM-67175 Comparative genome-wide analysis of small RNAs of major Gram-positive pathogens from identification to application In the recent years, the number of drug- and multi-drug-resistant microbial strains has increased rapidly. Therefore, the need to identify innovative approaches for development of novel anti-infectives and new therapeutic targets is of high priority in global health care. The detection of small RNAs (sRNAs) in bacteria has attracted considerable attention as an emerging class of new gene expression regulators. Several experimental technologies to predict sRNA have been established for the Gram-negative model organism Escherichia coli. In many respects, sRNA screens in this model system have set a blueprint for the global and functional identification of sRNAs for Gram-positive microbes, but the functional role of sRNAs in colonization and pathogenicity for Listeria monocytogenes, Staphylococcus aureus, Streptococcuspyogenes, Enterococcus faecalis and Clostridium difficile is almost completely unknown. Here, we report the current knowledge about the sRNAs of these socioeconomically relevant Gram-positive pathogens, overview the state-of-the-art high-throughput sRNA screening methods and summarize bioinformatics approaches for genome-wide sRNA identification and target prediction. Finally, we discuss the use of modified peptide nucleic acids (PNAs) as a novel tool to inactivate potential sRNA and their applications in rapid and specific detection of pathogenic bacteria. anti-infectiveness peptide nucleic acids sRNA antiinfektivnost peptidne nukleinske kisline sRNA true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-08-04 13:22:43 2017-08-04 13:23:56 2022-08-01 13:38:26 2019-07-11 16:11:30 2010 0 0 str. 658-676 št. 6 Letn. 3 2010 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1751-7915 579 14597398 512101944 10.1111/j.1751-7915.2010.00171.x 1751-7915 URN:SI:UM:DK:ID7ZCTRU Microbial_Biotechnology_2010_Mraheil_et_al._Comparative_genome-wide_analysis_of_small_RNAs_of_major_Gram-positive_pathogens_from_identif.pdf Microbial_Biotechnology_2010_Mraheil_et_al._Comparative_genome-wide_analysis_of_small_RNAs_of_major_Gram-positive_pathogens_from_identif.pdf 1 9373F551AB779D06F96B5F7E7B99B43C f1bd96b4706e99c01378fa706e1c4f4b1dbc1992e1ba7c1f20ae0c966ce35b8f 7b7907f4-7c0e-11eb-bb7a-00155d0001ca 20.500.12556/dkum/ec817760-9646-4eab-8181-91f8e3f268e1 https://dk.um.si/Dokument.php?lang=slv&id=114879 http://doi.wiley.com/10.1111/j.1751-7915.2010.00171.x 1 https://dk.um.si/Dokument.php?lang=slv&id=114878 Medicinska fakulteta 0 0 0