20.500.12556/DKUM-67640 Simultaneous GC-MS determination of free and bound phenolic acids in Slovenian red wines and chemometric characterization Several phenolic acids (PAs), caffeic, vanillic, syringic, p-coumaric and ferulic acid, found in Slovenian red wines were studied using gas chromatography and mass spectrometry (GC-MS). For isolation of the PAs from wine samples, solid phase extraction (SPE) using hydrophilic modified styrene - HLB cartridges was used. The bound PAs were extracted after basic hydrolysis and o-coumaric acid was used as the internal standard (ISTD). The method developed was validated and the linear concentration range for all analytes was from 1 to 100 mg L$^{-1}$ with correlation coefficients above 0.999. We show that the method is repeatable (RSD<2%), recoveries were above 96%, and LOD and LOQ values were acceptable. In all of the wine samples tested, caffeic and p-coumaric acid were determined to be the predominant PAs (17-72 mg L$^{-1}$), while other compounds were found in lower concentrations. Principal Component Analysis (PCA) and Cluster Analysis (CLU) were used to study differences between wines related towards varieties and Slovenian wine regions. The results demonstrate that variety has more influence on PAs content than wine regions in Slovenian red wines. Izbrane fenolne kisline: kavno, vanilinsko, siringinsko, kumarinsko in ferulno smo v različnih rdečih slovenskih vinih analizirali z uporabo GC-MS. Za izolacijo smo uporabljali ekstrakcijski postopek na HLB SPE kolonicah, ki vsebuje hidrofilno modificiran stiren. Pri delu smo vezane fenolne kisline hidrolizirali, kot interni standard pa smo uporabljali orto-kumarinsko kislino. Razvito metodo smo validirali: linearno koncentracijsko območje fenolnih kislin je med 1 in 100 mg L$^{-1}$, korelacijski koeficienti so bili nad 0,999. Potrdili smo dobro ponovljivost, RSD je znašal pod 2%, izkoristke nad 96% in sprejemljive vrednosti LOD ter LOQ. Ugotovili smo, da sta v vzorcih slovenskih vin prevladujoči fenolni spojini kavna in para-kumarna kislina (17–72 mg L$^{-1}$), medtem ko ostale spojine najdemo v nižjem koncentracijskem območju. Metodo glavnih osi (PCA) in analizo klastrov (CLU) smo uporabili za študij podobnosti in razlik med vzorci glede vsebnosti in deležev fenolnih kislin. Potrdili smo korelacijo in večji vpliv sorte grozdja kot vinorodne dežele oziroma regije, iz katere vzorci vin izvirajo. phenolic acids Slovenian red wines gas chromatography mass spectrometry PCA CLU fenolni acidi slovenska rdeča vina plinska kromatografija masna spektrometrija true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-08-25 10:52:56 2017-08-25 10:53:50 2022-08-01 14:16:23 2019-07-11 16:26:29 2016 0 0 str. 661-669 št. 3 Letn. 63 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1318-0207 66 14086149 19728662 10.17344/acsi.2016.2534 1318-0207 URN:SI:UM:DK:UMTW2EKZ Acta_Chimica_Slovenica_2016_Ivanovic,_Islamcevic_Razborsek,_Kolar_Simultaneous_GC-MS_Determination_of_Free_and_Bound_Phenolic_Acids_in_S.pdf Acta_Chimica_Slovenica_2016_Ivanovic,_Islamcevic_Razborsek,_Kolar_Simultaneous_GC-MS_Determination_of_Free_and_Bound_Phenolic_Acids_in_S.pdf 1 4737ED92FC39808498566BF744EAE062 9a5ddae2f32da0a1856c590e911ccf41ee78570272ad1f553a93623cd5145c90 8fde5455-7c0e-11eb-bb7a-00155d0001ca 20.500.12556/dkum/74c16350-14a1-4185-89e4-7fe0f9b35545 https://dk.um.si/Dokument.php?lang=slv&id=116038 https://journals.matheo.si/index.php/ACSi/article/view/2534 1 https://dk.um.si/Dokument.php?lang=slv&id=116037 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0