20.500.12556/DKUM-68962 Identification of hypothetical duplicate accessions of plums (Prunus domestica L.) within the Slovene Plant Gene Bank Collection using molecular markers Identifikacija morebitnih duplikatov med akcesijami sliv (Prunus domestica L.) znotraj Slovenske rastlinske genske banke z uporabo molekulskih markerjev The main goal of the Slovene Plant Gene Bank is preservation, maintenance and evaluation of traditional cultivars and other useful genotypes. The Faculty of Agriculture and Life Sciences houses among other plant materials also numerous accessions of plums (Prunus domestica L.). Duplicates among 15 accessions were studied using six microsatellite primer pairs. These microsatellite markers revealed an average of 7.67 alleles per locus, and a range of 4 to 10 different alleles per locus. The genetic distances between studied accessions were calculated using the Dice coefficient to form a dendrogram. The six SSRs were found to be adequate for differentiating among genotypes within the collection. Among the analysed accessions no duplicates were found. Slovenska rastlinska genska banka skrbi za ohranjanje, vzdrževanje in vrednotenje tradicionalnih kultivarjev in drugih uporabnih genotipov rastlin. Fakulteta za kmetijstvo in biosistemske vede skrbi med drugim tudi za številne akcesije sliv (Prunus domestica L.). Med 15 izbranimi akcesijami, smo s pomočjo šestih mikrosatelitskih lokusov želeli poiskati morebitne duplikate. Z mikrosatelitskimi markerji smo v povprečju namnožili 7.67 alelov na lokus in dobili od 4 do 10 različnih alelov na posamezen lokus. Za računanje genetskih oddaljenosti med proučevanimi akcesijami smo izračunali Dice koeficient in izrisali dendrogram. Šest mikrosatelitskih lokusov je zadostovalo za razlikovanje med akcesijami. Med preučevanimi akcesijami nismo našli duplikatov. gene bank duplicate accessions Prunus domestica plums SSR markers genska banka podvojene akcesije Prunus domestica slive mikrosatelitski markerji true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-11-14 12:29:21 2017-11-14 12:30:17 2022-08-01 16:31:50 2019-07-11 17:16:35 2016 0 0 str. 57-64 št. 1-2 Letn. 13 December 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1580-8432 631.52:634.22 116855808 4310060 10.1515/agricultura-2017-0007 1580-8432 URN:SI:UM:DK:Y9NYASLT Agricultura_2016_Sisko_Identification_of_hypothetical_duplicate_accessions_of_plums_(Prunus_domestica_L.)_within_the_slovene_plant_gene.pdf Agricultura_2016_Sisko_Identification_of_hypothetical_duplicate_accessions_of_plums_(Prunus_domestica_L.)_within_the_slovene_plant_gene.pdf 1 06AAB0653254E97F139ED61176ED1111 cd42ad9862e6c81d4a1bc03e48e51c6fae6dd049cd088e0a7a5d0d203590b404 7ed6a473-7c0f-11eb-bb7a-00155d0001ca 20.500.12556/dkum/ad44ea7a-f331-4472-9c3b-bc73c9dde11c https://dk.um.si/Dokument.php?lang=slv&id=120269 http://www.degruyter.com/view/j/agricultura.2016.13.issue-1-2/agricultura-2017-0007/agricultura-2017-0007.xml 1 https://dk.um.si/Dokument.php?lang=slv&id=120268 Fakulteta za kmetijstvo in biosistemske vede Agricultura 0 0 0