20.500.12556/DKUM-68965 In vitro tissue culture initiation from potted and garden Hydrangea macrophylla explants Iniciacija rastlinske tkivne kulture lončne in vrtne hortenzije (Hydrangea macrophylla) v in vitro razmerah The effect of sterilisation treatments on the initiation of the culture of three Hydrangea macrophylla genotypes in vitro was studied. The results indicated difference among different disinfection treatment; the consequence of treatments with dichloroisocyanuric acid, in combination with silver nitrate, was a significantly higher survival rate comparing to treatments based on NaOCl. Success of sterilisation was significantly influenced by studied genotypes. Two potted hydrangea genotypes (G2 and G3) showed higher survival rate (45.00 %, 55.00 %, respectively) comparing to the genotype 1 (originated from a local garden), which exhibited the highest percentage of contamination rate (58.75 %). Culturing the explants on a McCown woody plant medium supplemented with BAP at 2 mg/L and NAA at 0.005 mg/L produced the highest number of shoots with multiplication rate of 1.31 in 5 weeks. The highest rooting percentage (100.00 %) was obtained with the genotype 2 on a McCown woody plant medium supplemented with 0.5 mg/L IBA. Proučevali smo vpliv različnih načinov sterilizacije na uspešno vzpostavitev tkivne kulture treh genotipov hortenzije (Hydrangea macrophylla). Ugotovili smo, da obstajajo razlike med različnimi načini sterilizacije. Sterilizacija z dikloroizocianurno kislino v kombinaciji s srebrovim nitratom je bila uspešnejša od sterilizacije z uporabo NaOCl. Na uspešnost sterilizacije je vplival tudi genotip. Lončne hortenzije (genotipa G2 in G3) so kazale večjo sposobnost sterilizacije (pri genotipu G2 smo tako dobili 45.00 %, pri G3 pa 55.00 % uspešno steriliziranih poganjkov) v primerjavi z vrtno hortenzijo, kjer je bilo največ okuženih poganjkov (58.75 %). Za namnoževanje poganjkov se je kot najboljše izkazalo gojišče McCown z dodatkom 2 mg/L BAP in 0.005 NAA, na katerem je bil faktor namnoževanja 1,31 v petih tednih. Najbolje je koreninil genotip 2 na McCown osnovnem gojišču z dodano 0,5 mg/L IBA in sicer je na tem gojišču koreninilo 100 % poganjkov. micropropagation tissue culture hydrangea Hydrangea macrophylla plant disinfection mikropropagacija tkivne kulture hortenzija Hydrangea macrophylla sterilizacija rastlin true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-11-14 12:41:46 2017-11-14 12:42:36 2022-08-01 16:32:02 2019-07-11 17:16:41 2016 0 0 str. 65-69 št. 1/2 Letn. 13 December 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1580-8432 631.526:582.785 116855808 4309804 10.1515/agricultura-2017-0008 1580-8432 URN:SI:UM:DK:Q4BZEHYQ Agricultura_2016_Sisko_In_vitro_tissue_culture_initiation_from_potted_and_garden_Hydrangea_macrophylla_explants.pdf Agricultura_2016_Sisko_In_vitro_tissue_culture_initiation_from_potted_and_garden_Hydrangea_macrophylla_explants.pdf 1 5725D3F2B8026465544872A6B219A7C3 ab14e292d067371fc94594dd7b50a1cbecc9a42a37fca6d99b486641c120295e 7f018f67-7c0f-11eb-bb7a-00155d0001ca 20.500.12556/dkum/78bbc32c-2ff7-4d00-a55e-fee54243bd70 https://dk.um.si/Dokument.php?lang=slv&id=120275 http://www.degruyter.com/view/j/agricultura.2016.13.issue-1-2/agricultura-2017-0008/agricultura-2017-0008.xml 1 https://dk.um.si/Dokument.php?lang=slv&id=120274 Fakulteta za kmetijstvo in biosistemske vede Agricultura 0 0 0