20.500.12556/DKUM-69225 Low overlap between carbapenem resistant Pseudomonas aeruginosa genotypes isolated from hospitalized patients and wastewater treatment plants The variability of carbapenem-resistant Pseudomonas aeruginosa strains (CRPA) isolated from urine and respiratory samples in a large microbiological laboratory, serving several health care settings, and from effluents of two wastewater treatment plants (WWTP) from the same region was assessed by PFGE typing and by resistance to 10 antibiotics. During the 12-month period altogether 213 carbapenem-resistant P. aeruginosa isolates were cultured and distributed into 65 pulsotypes and ten resistance profiles. For representatives of all 65 pulsotypes 49 different MLSTs were determined. Variability of clinical and environmental strains was comparable, 130 carbapenem-resistant P. aeruginosa obtained from 109 patients were distributed into 38 pulsotypes, while 83 isolates from WWTPs were classified into 31 pulsotypes. Only 9 pulsotypes were shared between two or more settings (hospital or WWTP). Ten MLST were determined for those prevalent pulsotypes, two of them (ST111 and ST235) are among most successful CRPA types worldwide. Clinical and environmental carbapenem-resistant P. aeruginosa strains differed in antibiotic resistance. The highest proportion of clinical isolates was resistant to piperacillin/tazobactam (52.3%) and ceftazidime (42.3%). The highest proportion of environmental isolates was resistant to ceftazidime (37.1%) and ciprofloxacin (35.5%). The majority of isolates was resistant only to imipenem and/or meropenem. Strains with additional resistances were distributed into nine different patterns. All of them included clinically relevant strains, while environmental strains showed only four additional different patterns. carpabenem resistance antibiotic resistance Pseudomonas aeruginosa isolates odpornost proti karpabenemom odpornost proti antibiotikom Pseudomonas aeruginosa izolati true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-12-12 08:43:46 2017-12-12 08:44:57 2022-08-01 17:03:03 2019-07-11 17:25:18 2017 0 0 str. 1-13 št. 10 Letn. 12 2017 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1932-6203 579.61 2005896 512751672 10.1371/journal.pone.0186736 1932-6203 URN:SI:UM:DK:EAKSFZKP PLOS_ONE_2017_Golle,_Janezic,_Rupnik_Low_overlap_between_carbapenem_resistant_Pseudomonas_aeruginosa_genotypes_isolated_from_hospitalize.pdf PLOS_ONE_2017_Golle,_Janezic,_Rupnik_Low_overlap_between_carbapenem_resistant_Pseudomonas_aeruginosa_genotypes_isolated_from_hospitalize.pdf 1 981A40A1145757F001D31DD89EF720E4 a452c3c230e97253c19fcd062bc9d7b3d215b58b515c155dd5bfa72d23cf74dd 8e9b4ea6-7c0f-11eb-bb7a-00155d0001ca 20.500.12556/dkum/a3cc2d27-c952-4d7f-9cf9-f1c21d0e0e3b https://dk.um.si/Dokument.php?lang=slv&id=120908 http://dx.plos.org/10.1371/journal.pone.0186736 1 https://dk.um.si/Dokument.php?lang=slv&id=120907 Medicinska fakulteta 0 0 0