20.500.12556/DKUM-69227 Evaluating the effect of Clostridium difficile conditioned medium on fecal microbiota community structure Clostridium difficile infection (CDI) is typically associated with disturbed gut microbiota and changes related to decreased colonization resistance against C. difficile are well described. However, nothing is known about possible effects of C. difficile on gut microbiota restoration during or after CDI. In this study, we have mimicked such a situation by using C. difficile conditioned medium of six different C. difficile strains belonging to PCR ribotypes 027 and 014/020 for cultivation of fecal microbiota. A marked decrease of microbial diversity was observed in conditioned medium of both tested ribotypes. The majority of differences occurred within the phylum Firmicutes, with a general decrease of gut commensals with putative protective functions (i.e. Lactobacillus, Clostridium_XIVa) and an increase in opportunistic pathogens (i.e. Enterococcus). Bacterial populations in conditioned medium differed between the two C. difficile ribotypes, 027 and 014/020 and are likely associated with nutrient availability. Fecal microbiota cultivated in medium conditioned by E. coli, Salmonella Enteritidis or Staphylococcus epidermidis grouped together and was clearly different from microbiota cultivated in C. difficile conditioned medium suggesting that C. difficile effects are specific. Our results show that the changes observed in microbiota of CDI patients are partially directly influenced by C. difficile. Clostridium difficile infection gut microbiota Clostridium difficile infekcija črevesna mikrobiota črevesna flora true false true Angleški jezik Slovenski jezik Znanstveno delo 2017-12-12 09:51:24 2017-12-12 09:52:37 2022-08-01 17:03:11 2019-07-11 17:25:22 2017 0 0 str. 1-11 št. 1 Letn. 7 2017 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2045-2322 579.852.13 18727432 512751416 10.1038/s41598-017-15434-1 2045-2322 URN:SI:UM:DK:3AKXLLXR Scientific_Reports_2017_Horvat_et_al._Evaluating_the_effect_of_Clostridium_difficile_conditioned_medium_on_fecal_microbiota_community_st.pdf Scientific_Reports_2017_Horvat_et_al._Evaluating_the_effect_of_Clostridium_difficile_conditioned_medium_on_fecal_microbiota_community_st.pdf 1 D00136E00D21FAFC9BFE74827C11431F 82b543930701c8d4c68bdc669b7d399cd7c68d9cc29587b93cc14bbd95005e7f 8eb7f633-7c0f-11eb-bb7a-00155d0001ca 20.500.12556/dkum/514a7e67-eb62-4806-b683-8ef6b943ae06 https://dk.um.si/Dokument.php?lang=slv&id=120912 http://www.nature.com/articles/s41598-017-15434-1 1 https://dk.um.si/Dokument.php?lang=slv&id=120911 Medicinska fakulteta 0 0 0