20.500.12556/DKUM-70117 Uporabnost aparature morapex a v pralnici Applicability of the morapex a device in the laundry Izhodišče: Bolnišnično okolje predstavlja veliko nišo za razvoj patogenih organizmov. Med vektorje prenosa mikroorganizmov sodijo tudi tekstilije, ki so v negi pacienta z njim v neposrednem kontaktu. Zato je pomembno zagotavljanje ustrezne kontrole kakovosti postopkov razkuževalnega procesa tekstilij. Tako se razvijejo nove optimalne rešitve detekcije in identifikacije patogenih mikroorganizmov, ki morajo biti hitre, dostopne, cenovno ugodne, hkrati pa tudi nedestruktivne. Metodologija: V raziskavi smo izvedli vzorčenje v pralnici na kritičnih kontrolnih točkah. Vzorčili smo 5 tekstilij na petih različnih mestih. Tekstilije smo zaradi sledljivosti označili s pomočjo pralne vreče. Vzorčenje je bilo izvedeno z aparatom Morapex A z nedestruktivno metodo eluiranja. Eluate smo v laboratoriju aseptično nanesli na RODAC agar plošče in po inkubaciji prešteli število kolonij in ocenili morfološki izgled kolonij Pri tem smo ugotavljali uporabnost aplikacije Morapex A aparata za kontrolo kakovosti pralnega procesa. Rezultati: Ugotovili smo, da se s pranjem v pralnici zagotovi ustrezna higiena tekstilij, v kolikor ne pride do ponovne rekontaminacije. Slednja je najpogostejša po stiku opranega perila z osebjem pralnice. Pogostejše vzorčenje z Morapex A napravo doprinese k hitrim odkrivanjem morebitnih kontaminacij pralne linije. V času raziskave se je izkazalo, da aplikacija naprave Morapex A za uporabo monitoringa higiene tekstilij le delno nadomešča uveljavljene metode, saj je vzorčenje z njo nedestruktivno in tako omogoča sledljivost vzorca, pri čemer je metoda omejena z vrsto tekstilije in števnostjo vzorcev. Sklep: Z uvedbo Morapex A aparature v kontrolo kakovosti pralnega procesa bi izboljšali sledljivost vzorca, zaradi nedestruktivnosti metode pa povečali tudi pogostost vzorčenja. Starting point: The hospital environment represents great opportunities for pathogenic organisms to develop. Vectors of microbial transmission include textiles, which are in direct contact with the patient during their care. It is therefore important to ensure proper quality control of disinfection process for textiles. New optimal solutions for detection and identification of pathogenic microorganisms are developed and they have to be quick, accessible, affordable and non-destructive at the same time. Methodology: We took different samples in a laundry during our research. Sampling 5 samples at 5 critical control points was carried out in the study. Textiles were labelled with a wash bag for easier tracking. Samples were taken with Morapex A apparatus with non-destructive elution method. Samples were aseptically applied to RODAC agar plates and after the incubation we counted the number of colonies. With that we determined how useful the application of Morapex A apparatus is for quality control process. Results: We found out that appropriate hygiene of textiles is ensured by washing in laundry, as long there is no recontamination. This is more frequent after contact of laundry staff with washed textiles. More frequent sampling with Morapex A apparatus contributes to rapid detection of possible contamination of washing line. During the research we have proved that application of Morapex A apparatus for the use of textile hygiene monitoring only partially substitutes established methods. Sampling with this method is non-destructive and thus enables the traceability of the sample, but is limited by textile type and sample count. Conclusion: The introduction of Morapex A apparatus in the quality control of the washing process would improve the traceability of the sample, and due to the non-destructive method, the sampling rate could also be increased. bolnišnične okužbe pralni proces higiena tekstilij RODAC agar plošče nedestruktivna metoda. hospital infections washing process hygiene of textiles RODAC agar plates non-destructive method. true false false [T. Mikluš] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2018-04-09 19:35:13 2018-07-13 13:40:22 2022-08-01 18:49:30 2019-07-11 17:56:28 2018 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 648:579.61:616-092(043.2) 2421924 URN:SI:UM:DK:PMHIFQFG MAG_Miklus_Tjasa_2018.pdf MAG_Miklus_Tjasa_2018.pdf 1 8A536A8BE6A4830FBE16E51A193CAEE0 216e1cdd0eedbc357d406e4e8915b45a14c999d3f8e5e708f83ea33b3af3ea8f de32d362-7c0f-11eb-bb7a-00155d0001ca 20.500.12556/dkum/d4034808-8187-405e-a3b9-e48c1f8d6457 https://dk.um.si/Dokument.php?lang=slv&id=123713 Fakulteta za zdravstvene vede 0 0 0