20.500.12556/DKUM-70721 Zaščitno delovanje izbranih polifenolnih snovi proti genotoksičnim učinkom, ki jih povzroča mikotoksin aflatoksin B1 The protective effects of selected polyphenolic substances against genotoxicity induced by micotoxin aflatoxin B1 Namen diplomskega dela je bil raziskati morebitno antigenotoksično delovanje naravnih polifenolnih snovi, natančneje ksantohumola (XN), proti genotoksičnim učinkom mikotoksina aflatoksina B1 (AFB1), kar smo ugotavljali posredno preko merjenja fosforilacije histona H2AX (znan marker dvoverižnih prelomov DNA) s pretočnim citometrom na modelu metabolno aktivnih celic človeškega hepatoma HepG2. Iz literature je znano da XN, prenilirani flavonoid izoliran iz ženskih cvetov hmelja, deluje zaščitno pred poškodbami DNA, ki jih povzročajo nekateri heterociklični aromatski amini, kar kaže na njegovo potencialno uporabnost v obliki prehranskega ali farmacevtskega dopolnila. Rezultati diplomske naloge so pokazali, da sam XN pri najvišji testirani koncentraciji 10 µM ne povzroča povečanja žarišč H2AX pri izpostavljenih celicah HepG2, med tem ko je AFB1 od doze značilno povišal število H2AX žarišč, ki se je od kontrolne skupine statistično značilno razlikovalo že pri najnižji testirani koncentraciji (10 µM) . Zaščitni učinek XN zoper z AFB1 (30 µM) povzročenim žariščem H2AX smo zaznali pri dveh najvišjih testiranih koncentracijah XN (1 in 10 µM), in sicer od doze odvisno. Rezultati diplomske naloge nakazujejo, da XN deluje zaščitno proti genotoksičnim učinkom AFB1 pri celicah HepG2, kar sovpada s podatki iz literature, ki kažejo, da XN poseduje želene antigenotoskične lastnosti. Z eksperimentalnimi podatki smo podkrepili hipotezo, da ima XN zaščitni učinek pred genotoksičnimi učinki AFB1. The purpose of this thesis was to assess the possible antigenotoxic activity of natural polyphenolic substances, more precisely xanthohumol (XN), against the genotoxic effects of mycotoxin aflatoxin B1 (AFB1), which was determined indirectly through the measurement of phosphorylation of histone H2AX (known marker of DNA double breaks) with a flow cytometer on the model metabolically active human hepatoma HepG2 cells. It is known from the literature that XN, a prenylated flavonoid isolated from female flowers of hops, protects against DNA damage induced by some heterocyclic aromatic amines, indicating its potential applivabolity in the form of a nutritional or pharmaceutical supplement. The results of this thesis showed that XN alone did not cause H2AX foci in exposed HepG2 cells even at the highest tested concentration of 10 μM, while AFB1 statistically increases the number of H2AX foci already at the lowest tested concentration (10 μM) compared to untreated HepG2 cells. On the basis of these results 30 μM AFB1 was selected for the testing of possible antigenotix effeects of XN. The protective effect of XN against the formation of H2AX foci induced by AFB1 (30 μM) was detected at two highest tested concentrations of 1 and 10 μM, which was dose dependent. These results indicated that XN represents a protective agent against the genotoxic effects induced by AFB1 in HG2 cells, which corroborates the literature data showing that XN possesses the desired antigenotoxic properties. With experimental data we supported the hypothesis that XN exerts a protective effect against the genotoxic activity of AFB1. aflatoksin B1 ksantohumol celice HepG2ATTC pretočni citometer Dvoverižni prelomi DNA aflatoxin B1 xanthohumol HepG2ATTC cells flow cytometer Double Strand Breaks DNA true true false [M. Roškarič] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2018-06-10 20:46:26 2018-10-04 14:09:59 2022-08-01 19:43:53 2019-07-11 18:17:35 2018 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 547.568.5(043.2) 21962774 URN:SI:UM:DK:P1LXE27H UN_Roskaric_Matevz_2018.pdf UN_Roskaric_Matevz_2018.pdf 1 6FA4F1EAEE8F02338F2B5BDA4BDD707D 5c094e4439d0c58529cdaecca1871d414bfffe420d043396161a67d4ecffe7f0 500e0256-7c10-11eb-bb7a-00155d0001ca 20.500.12556/dkum/c2e24e78-c86f-4bdf-a54e-872a4c875810 https://dk.um.si/Dokument.php?lang=slv&id=129352 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0