20.500.12556/DKUM-71023 Aktivnost lakaze in celulaze v glivi Pleurotus ostreatus Activity of laccase and cellulase in the fungus Pleurotus ostreatus Diplomsko delo opisuje gojenje glive Pleurotus ostreatus (P. ostreatus) na trdnem gojišču ob uporabi ustreznega rastnega substrata. Opazovali smo prirast glive in merili njegovo maso v odvisnosti od časa inkubacije. Po določenem časovnem obdobju smo izvedli ekstrakcijo encimov ter določili aktivnosti encima lakaze in celulaze v teh vzorcih. Zanimal nas je vpliv časa inkubacije na aktivnosti encimov. Prav tako smo določali aktivnost encimov iz zamrznjenih encimskih ekstraktov, saj smo želeli ugotoviti, kako se spremeni aktivnost encima, če ustvarimo mešanico koncentriranega encimskega ekstrakta. Diplomsko delo prav tako opisuje zamreženje encimskih skupkov (CLEAs) iz encimskih ekstraktov. Ta postopek smo izvedli po določenem protokolu z uporabo različnih obarjalnih reagentov (aceton, etanol, 1-propanol, 2-propanol) in z mrežnim povezovalcem glutaraldehidom (GA). Zamreženim encimskim skupkom smo določili učinkovitost imobilizacije, preostalo aktivnost in preostalo specifično aktivnost. Z namenom, da bi dosegli boljše rezultate, smo spremenili volumske deleže mrežnega povezovalca GA, obarjali pa smo le v acetonu, ker se je izkazalo, da je obarjanje encimskega preparata bilo najuspešnejše z njegovo uporabo. Zamreževanje je potekalo 3 ure na sobni temperaturi. The diploma work describes the cultivation of the fungus P. ostreatus on a solid medium using an appropriate growing substrate. We observed growth in fungi and measured its mass depending on the incubation time. After defined period of time, extraction of enzymes was carried out and the activity of the enzyme laccase and cellulase in the samples was determined. We were interested in the influence of incubation time on enzyme activity. We also determined the activity from frozen enzyme extracts as we wanted to determine how the enzyme activity was changed if a mixture of concentrated enzyme extract. The diploma work also describes the cross-linked enzyme aggregates (CLEAs) from enzyme extracts. This procedure was carried out according to a particular protocol using various precipitation reagents (acetone, ethanol, 1-propanol, 2-propanol) and with cross-linker glutaraldehyde (GA). Immobilization efficiency, residual activity, and residual specific activity of cross-linked enzyme aggregates was determined. In order to achieve better results, we changed the volume fraction of the cross-linker glutaraldehyde and precipitated only in acetone, as the precipitation of the enzyme preparation proved to be the most successful with its use. The cross-linking was carried out at room temperature for 3 hours. Pleurotus ostreatus lakaza celulaza aktivnost imobilizacija glutaraldehid Pleurotus ostreatus laccase cellulase activity immobilization glutaraldehyde true true false [M. Taljan] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2018-07-02 08:53:36 2018-07-23 10:17:08 2022-08-01 20:16:30 2019-07-11 18:27:35 2018 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 582.28:543.383(043.2) 21606678 URN:SI:UM:DK:J7AIPKGV VS_Taljan_Mihaela_2018.pdf VS_Taljan_Mihaela_2018.pdf 1 0C5762229DC1C9F85A5C1A01EBFBBFF4 bc1d182312550c71fb36455d5ffbf7b117b63f7fe0a7e2f341b1de0ab70bb7cf 1d1c4155-7c10-11eb-bb7a-00155d0001ca 20.500.12556/dkum/f0a48e40-57d7-4463-87c7-3d12ae7b2d45 https://dk.um.si/Dokument.php?lang=slv&id=126603 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0