20.500.12556/DKUM-71919 Strukturne značilnosti celic alfa Langerhansovih otočkov miši Structural characteristic in alpha cells of mouse langerhans islets V tej raziskavi je bil naš namen pregledati in primerjati ultrastrukturo celic alfa v Langerhansovih otočkih, v treh makroskopsko razdeljenih delih (spleničnem, gastričnem in duodenalnem delu) trebušne slinavke miši. Opazovali in primerjali smo velikosti glukagonskih veziklov in granul v tkivu, ki je bilo izpostavljeno različnim koncentracijam glukoze v različno dolgih časovnih intervalih. Vzorce tkiva smo analizirali s svetlobno mikroskopijo in presevno elektronsko mikroskopijo (TEM). Dobljene podatke smo statistično ovrednotili. Ugotovili smo, da je ultrastruktura organelov celic alfa v različnih delih trebušne slinavke in v tkivu, izpostavljenemu različnim koncentracijam glukoze, primerljiva. Statistično značilne razlike so bile opazne v premerih granul in veziklov ter v površini veziklov med tremi različnimi deli trebušne slinavke. Vzorci, izpostavljeni različnim koncentracijam glukoze v različno dolgih časovnih intervalih, so se statistično razlikovali v premerih granul in veziklov, v površini granul in veziklov ni bilo statističnih razlik. Med premeri in deleži površin granul in veziklov ob plazmalemi, ki so bili izpostavljeni različnim koncentracijam glukoze v različnem času, ni bilo statističnih razlik. Za nadaljnje raziskave bi bilo treba vključiti večje število Langerhansovih otočkov z vsebovanimi tipi celic, obravnavane tkivne rezine bi lahko bile izpostavljene še višjim koncentracijam glukoze in za daljše časovno obdobje. In this study, our purpose was to examine and compare the ultrastructure of alpha cells in the islets of Langerhans in three macroscopically divided parts (splenetic, gastric and duodenal region) of the pancreas in mice. We observed and compared the size of glucagon vesicles and granules in the tissue, which was exposed to different glucose concentrations at various time intervals. With light microscopy and transmission electron microscopy (TEM) we analyzed tissue samples. The obtained data were statistically evaluated. We established that the ultrastructure of alpha cells organelles is similar in different parts of the pancreas and at various concentrations of glucose. Statistically significant differences were observed in the diameter of the granules and vesicles, and in the surface of the vesicles between the three different parts of the pancreas. Samples, exposed to different glucose concentrations at various time intervals, statistically differed in the diameter of the granules and vesicles, but no statistical differences were seen in the surface of granules and vesicles. Among the diameters and surface percentage of granules and vesicles near the cell membrane, which were exposed to different glucose concentrations at various time intervals, no statistical differences were seen. For further research, it would be necessary to include a larger number of islets with the contained cell types. Additionally, the treated tissue slices could be exposed to higher concentrations of glucose and for a significant period of time. glukagon glukoza granule miš trebušna slinavka ultrastruktura vezikli glucagon glucose granules mouse pancreas ultrastructure vesicles true true false [M. Prosenica] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2018-09-04 11:36:21 2018-11-20 14:32:39 2022-08-01 21:44:26 2019-07-11 18:59:25 2018 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 591.437:599.323.451(043.2) 24118024 URN:SI:UM:DK:JJOY4MGO MAG_Prosenica_Mia_2018.pdf MAG_Prosenica_Mia_2018.pdf 1 332C222C43BCB1AE9FBEB21462D3DC0D a85cfb0674e00755f010f85683b5d1ed616cdd7295c79765058b9666dcbcc6bd 3fdd1114-7c10-11eb-bb7a-00155d0001ca 20.500.12556/dkum/a03292e0-0b70-4a1d-b1dd-0767b489a79e https://dk.um.si/Dokument.php?lang=slv&id=128587 Fakulteta za naravoslovje in matematiko 0 0 0