20.500.12556/DKUM-72275 Ugotavljanje stresa konj s pomočjo neinvazivnih metod Monitoring stress in horses through non-invasive methods V raziskavi smo ugotavljali intenzivnost in trajanje izločanja stresnega hormona kortizola v slini konj, ob tem pa smo spremljali srčni utrip. Obe metodi veljata za neinvazivni. Delo s konji je potekalo od meseca februarja do konca maja 2018 na kmetiji Kolarič v Obrežu. V poskus smo vključili dva mlada in neujahana konja iz istega hleva – 3 leta staro žrebico slovenske hladnokrvne pasme ter 3 leta starega kastrata slovenske toplokrvne pasme. Sam poskus smo razdelili na predpreizkus in tri faze. V predpreizkusu je potekala habituacija, tako sta se konja navadila na merilno napravo ter sam postopek poskusa. V prvi fazi smo opravili le z lonžiranjem s hlevsko uzdo, v drugi fazi smo uvedli jahalno uzdo z navadno mehko brzdo, v tretji fazi pa še podsedelnico ter sedlo. Lonžiranje, ki je skupno trajalo 30 minut, smo izvajali na prostem, v ograjenem prostoru s travnato površino. Vzorci sline so v času posamezne vaje bili odvzeti 5-krat. Višje vrednosti srčnega utripa so se pojavljale pri hladnokrvnem konju ter so znašale 64,76 min-1, pri toplokrvnem konju 62,46 min-1. Večje razlike smo opazili pri izmerjenih vrednostih izločenega kortizola. Pri toplokrvnem konju so med izvajanjem vaje v prvi fazi znašale 0,105 µg/dl, v drugi 0,111 µg/dl, ter v tretji fazi 0,142 µg/dl. Pri hladnokrvnem pa so bile vrednosti med izvajanjem vaje v prvi fazi 0,076 µg/dl, v drugi 0,114 µg/dl ter v tretji fazi 0,122 µg/dl. The secretion intensity and the duration of the stress hormone cortisol in horse saliva, as well as their heart rate were measured. Both methods are considered non-invasive. The research lasted from February to the end of May 2018, on the Kolarič farm in Obrež. For the experiment we chose two young and untrained horses from the same barn, a 3 years old filly who is a Slovenian cold-blooded breed and a 3 years old castrated Slovenian warm-blooded breed. We divided the experiment into a pre-test and three phases. In the pre-test the horses were exposed to the procedure so that they could get used to the measuring equipment and then the procedure of the test. In the first phase we only did longeing with the barn rein. In the second phase we introduced a riding bridle with an ordinary soft bit and in the final third phase, a saddle pad and the saddle longeing which lasted 30 minutes. The research had taken place outdoors, in a fenced grassy area. Saliva samples from the horses were taken 5 times. A higher heart rate was recorded with the cold-blooded horse, but when the results we calculated, it showed a smaller difference. For the cold-blooded horse, the heart rate was 65 beats per min., and the warm blooded had a heart rate of 62 beats per minute. Bigger differences were recorded in the cortisol secretion. During the exercise, the warm-blooded horse had 0,105 µg/dl in the first phase, 0,111 µg/dl in the second, and 0,142 µg/dl in the third phase. The cold-blooded horse had 0,076 µg/dl in the first phase, 0,114 µg/dl in the second, and 0,122 µg/dl in the third phase. stres konji kortizol srčni utrip stress horses cortisol heart rate true true false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2018-09-17 22:51:53 2018-09-21 14:05:43 2022-08-01 22:23:05 2019-07-11 19:13:05 2018 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 URN:SI:UM:DK:P0UZMXGM VS_Plohl_Sergeja_2018.pdf VS_Plohl_Sergeja_2018.pdf 1 A300516BB2CE03819BCC4B530F808494 ad2f5468206280886df77e61be7d263dc6205a8fcbc53707c8821abb269fe7fc 585f7261-7c10-11eb-bb7a-00155d0001ca 20.500.12556/dkum/f07449c6-47a1-4fba-96e1-f28e1ccf45a2 https://dk.um.si/Dokument.php?lang=slv&id=129697 Fakulteta za kmetijstvo in biosistemske vede 0 0 0