20.500.12556/DKUM-72314 Razmnoževanje češnje (Prunus avium L.) v in vitro pogojih In vitro propagation of sweet cherries (Prunus avium L.) V letu 2016 smo na Fakulteti za kmetijstvo in biosistemske vede v Mariboru opravili poskus, pri katerem smo proučevali vpliv različnih načinov sterilizacije na brste dveh sort češenj. Za sterilizacijo smo uporabljali razkuževalni sredstvi DICA in natrijev hipoklorit. Kasneje smo sterilne in vitalne poganjke namnoževali na gojišču MS z dodanim citokininom (BAP) in avksinom (NAA). Ko smo namnožili dovolj poganjkov, smo preučevali gojišče za koreninjenje. Kontrolno gojišče je vsebovalo 0,0 mg/l IBA, gojišče G1 je vsebovalo 0,3 mg/l IBA in gojišče G2 je vsebovalo 1,0 mg/l IBA. Pri gojiščih G1 in G2 smo polovico poganjkov dali za 6 dni v temo. Po določenem času smo vrednotili število nastalih korenin. Najbolje se je izkazalo gojišče G2, ki je bilo izpostavljeno temi, kjer je bil uspeh koreninjenja 100 %, na gojišču G2, ki je bilo na svetlem, je bil uspeh koreninjenja 90 %. Na gojišču G1, ki je bilo na svetlem in temnem, je bil uspeh koreninjenja 60 %. Kontrolno gojišče je imelo 50 % uspeh koreninjenja. Največ korenin (povprečno 5,7 korenin na rastlino) so tvorili poganjki na gojišču G2, katere smo 6 dni tretirali s temo. Rastline, katere so razvile lepe korenine, smo na koncu aklimatizirali. In the year 2016, we carried out an experiment at the Faculty of Agriculture and Life Sciences of Maribor, in which we studied the effects of different types of sterilisation methods on buds of two cherry cultivars. Two disinfectants were used for sterilisation – DICA and sodium hypochlorite. Sterile and vital shoots were reproduced on a MS medium, where cytokinin BAP and auxin NAA were added. When we had enough reproduced shoots, we studied three types of rooting mediums. The control medium had 0.0 mg/l IBA, the medium G1 had 0.3 mg/l IBA and the medium G2 had 1 mg/l IBA. Half of the shoots, which were placed in medium G1 and G2, were treated with a dark treatment for six days. After a period, we determined the number of roots per plant. The best medium was the G2, which was exposed to darkness, in which the rooting was 100 %. In medium G2, which was exposed to light, the rooting was 90 %. The rooting in medium G1, which was treated with both, darkness and light, was 60 %. The rooting on control medium was 50 %. Most roots (on average 5.7 roots per plant) were grown by plants, which were grown in G2 medium, which was treated with six days of darkness. The plants that grew the best roots, were acclimatized. koreninjenje in vitro češnja tkivne kulture mikropropagacija rooting in vitro cherry tissue culture micropropagation true true false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2018-09-18 23:25:31 2018-10-02 12:03:19 2022-08-01 22:26:49 2019-07-11 19:14:42 2018 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 URN:SI:UM:DK:TPJ0QVE9 VS_Stamic_Domen_2018.pdf VS_Stamic_Domen_2018.pdf 1 6FE5906DFB4BC76513BC225F38942E97 c37c7fe6e4709c5c5ed78916000788ca1cb0f8516d94e276c65d2da6ffaaa293 5afc2ec8-7c10-11eb-bb7a-00155d0001ca 20.500.12556/dkum/8ef1d9b4-13ec-471e-b8d2-b4fb4b580669 https://dk.um.si/Dokument.php?lang=slv&id=129815 Fakulteta za kmetijstvo in biosistemske vede 0 0 0