20.500.12556/DKUM-73392 Določanje vsebnosti rastlinskim hormonom podobnih substanc v japonskem dresniku (Fallopia japonica) in kanadski zlati rozgi (Solidago canadensis) s pomočjo biotestov (Cucumis test) Determination of plant hormone-like substances of the japanese knotweed (Fallopia japonica) and canadian goldenrod (Solidago canadensis) with Cucumis bioassay Invazivne tujerodne vrste se hitro razmnožujejo in razširjajo ter izpodrivajo avtohtone vrste. V naši raziskavi smo želeli raziskati vsebnost rastlinskim hormonom podobnih substanc v dveh invazivnih tujerodnih rastlinskih vrstah, kanadski zlati rozgi (Solidago canadensis) in japonskem dresniku (Fallopia japonica). S kumaričnim (Cucumis) biotestom smo ugotavljali vsebnost avksinom in citokininom podobnih substanc v rastlinskem materialu, v različnih razvojnih stadijih rasti, sušenem v sušilniku ali liofilizatorju. V obeh proučevanih invazivnih vrstah smo v materialu, sušenem v liofilizatorju določili nekoliko več hormonom podobnih substanc v primerjavi s sušenim materialom v sušilniku. Kanadska zlata rozga (Solidago canadensis) je vsebovala med 65 µg in 116 µg avksinov in med 0 µg in 50 µg citokininov na gram suhe mase (SM) rastlin. Japonski dresnik (Fallopia japonica) je vseboval med 63 µg in 116 µg avksinov in med 0 µg in 53 µg citokininov na gram suhe mase (SM) rastline. Največ avksinov in avksinom podobnih substanc imata obe proučevani vrsti v drugem razvojnem stadiju, v katerem so rastline že razvile dolge, še neolesenele poganjke, ter liste in v tretjem razvojnem stadiju, v katerem so bile rastline skoraj popolnoma razvite, z že nekoliko olesenelimi stebli. Največ citokininov in citokininom podobnih substanc smo zaznali v drugem razvojnem stadiju pri obeh rastlinskih vrstah. The invasive alien species rapidly multiply, fast expand and thus displace the native species. The purpose of our thesis was determining the content of plant-hormone-like substances in two invasive alien plant species, the Canadian goldenrod (Solidago canadensis) and Japanese knotweed (Fallopia japonica). We used a cucumis (Cucumis) bioassay to establish the content of auxins-like and cytokinins-like substances in material in four different developmental stages, dryed in a dryer or freeze dryer. In both invasive species studied, there were more hormone-like substances in the material dried in the liofilizator than in a freeze dryer. The Canadian goldenrod (Solidago canadensis) contained between 65 µg and 116 µg of auxins-like and between 0 µg and 50 µg of cytokinins-like substances per gram of dry weight (DW). The Japanese knotweed (Fallopia japonica) contained between 63 µg and 116 µg of auxins-like and between 0 µg and 53 µg of cytokinins-like substances per gram of dry weight (DW). The content of auxins-like and cytokinins-like substances in both studied species was higher in the second growth stage in which the plants had already developed long, yet not lignificated shoots and leaves, as well as in the third developmental stage, in which the plants had been almost fully developed with slightly lignified stem. We noticed that the content of auxins-like and cytokinins-like substances was the highest in the second developmental stage of both plant species. Solidago canadensis kanadska zlata rozga Fallopia japonica japonski dresnik invazivne tujerodne rastline avksini indol-3-butanojska kislina (IBA) citokinini 6-benzilaminopurin (BAP) kumarični (Cucumis) biotest Solidago canadensis Canadian goldenrod Fallopia japonica Japanese knotweed invasive alien species auxins indol-3-butyric acid (IBA) cytokinins 6-benzylaminopurine (BAP) Cucumis Bioassay true true false [M. Luković] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2019-04-05 12:02:13 2019-08-29 13:39:46 2022-08-02 00:40:45 2019-08-31 03:12:45 2019 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.175.1:581.96(043.2) 24720136 URN:SI:UM:DK:NETN0TUI MAG_Lukovic_Maja_2019.pdf MAG_Lukovic_Maja_2019.pdf 1 2413C90844CE74472E1B45B40711F70C 0276de3c703ee4ef914939282a75bb7d1806d31a436560168ee4ed8475c5c43b dd9450ed-7c10-11eb-bb7a-00155d0001ca 20.500.12556/dkum/485cf942-ccc1-4846-952e-476421f32799 https://dk.um.si/Dokument.php?lang=slv&id=136652 Fakulteta za naravoslovje in matematiko 0 0 0